TY - JOUR
T1 - Growth of Ammonia-Oxidizing Archaea and Bacteria in Cattle Manure Compost under Various Temperatures and Ammonia Concentrations
AU - Oishi, Ryu
AU - Tada, Chika
AU - Asano, Ryoki
AU - Yamamoto, Nozomi
AU - Suyama, Yoshihisa
AU - Nakai, Yutaka
N1 - Funding Information:
This work was supported, in part, by the Foundation of the Ministry of Education, Culture, Sports, Science, and Technology, Japan, as a “Project of Integrated Compost Science” and by a grant from the Livestock Technology Association of Japan. We thank the technical staff of Tohoku University for providing compost samples. We would also like to thank Yasuhiro Fukuda and Hidenari Miyake for providing helpful comments on the manuscript.
PY - 2012/5
Y1 - 2012/5
N2 - A recent study showed that ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) coexist in the process of cattle manure composting. To investigate their physiological characteristics, liquid cultures seeded with fermenting cattle manure compost were incubated at various temperatures (37°C, 46°C, or 60°C) and ammonium concentrations (0. 5, 1, 4, or 10 mM NH4+-N). The growth rates of the AOB and AOA were monitored using real-time polymerase chain reaction analysis targeting the bacterial and archaeal ammonia monooxygenase subunit A genes. AOB grew at 37°C and 4 or 10 mM NH4+-N, whereas AOA grew at 46°C and 10 mM NH4+-N. Incubation with allylthiourea indicated that the AOB and AOA grew by oxidizing ammonia. Denaturing gradient gel electrophoresis and subsequent sequencing analyses revealed that a bacterium related to Nitrosomonas halophila and an archaeon related to Candidatus Nitrososphaera gargensis were the predominant AOB and AOA, respectively, in the seed compost and in cultures after incubation. This is the first report to demonstrate that the predominant AOA in cattle manure compost can grow and can probably oxidize ammonia under moderately thermophilic conditions.
AB - A recent study showed that ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) coexist in the process of cattle manure composting. To investigate their physiological characteristics, liquid cultures seeded with fermenting cattle manure compost were incubated at various temperatures (37°C, 46°C, or 60°C) and ammonium concentrations (0. 5, 1, 4, or 10 mM NH4+-N). The growth rates of the AOB and AOA were monitored using real-time polymerase chain reaction analysis targeting the bacterial and archaeal ammonia monooxygenase subunit A genes. AOB grew at 37°C and 4 or 10 mM NH4+-N, whereas AOA grew at 46°C and 10 mM NH4+-N. Incubation with allylthiourea indicated that the AOB and AOA grew by oxidizing ammonia. Denaturing gradient gel electrophoresis and subsequent sequencing analyses revealed that a bacterium related to Nitrosomonas halophila and an archaeon related to Candidatus Nitrososphaera gargensis were the predominant AOB and AOA, respectively, in the seed compost and in cultures after incubation. This is the first report to demonstrate that the predominant AOA in cattle manure compost can grow and can probably oxidize ammonia under moderately thermophilic conditions.
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U2 - 10.1007/s00248-011-9971-z
DO - 10.1007/s00248-011-9971-z
M3 - Article
C2 - 22072124
AN - SCOPUS:84864364819
VL - 63
SP - 787
EP - 793
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 4
ER -