Growth hormone release induced by an amino acid mixture from primary cultured anterior pituitary cells of goats

Y. Ohata, Y. Maruyama, Kazuo Katoh, Y. Sasaki

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    12 Citations (Scopus)


    The effects of amino acids on growth hormone (GH) release and cytosolic calcium concentration ([Ca2+](i)) were investigated in caprine anterior pituitary cells cultured for 3 d in Dulbecco modified Eagle medium. The addition of an amino acid mixture consisting of seven nonessential amino acids (NEAA: L-Asp, Gly, L-Ala, L-Ser, L-Pro, L-Asn, and L-Glu; concentration of each 12.5-200 μmol/l) in the medium significantly raised GH release from the cultured cells in a concentration-dependent manner with the maximum release at 200 μmol/l NEAA. Although an addition of L-Asp (0.1-100 μmol/l) caused a significant rise in GH release in a concentration-dependent manner, neither the individual amino acids contained in NEAA except L-Asp nor others (L-Leu, L-Phe, L-Gln, L-Met, and L-Arg) caused a rise in GH release when added alone to the medium. The rise in GH release induced by NEAA (200 μmol/l) and GH-releasing hormone (GHRH, 10 nmol/l) was significantly reduced by the addition of EGTA (1.8 mmol/l) and nifedipine (1 μmol/l) to the medium, respectively. The addition of NEAA (200 μmol/l) caused a rapid and transient [Ca2+](i) increase, followed thereafter by a steady increase. The prior addition of nifedipine (1 μmol/l), which itself significantly reduced the basal [Ca2+](i), completely abolished the response induced by NEAA or GHRH. From these findings, we conclude that: 1) NEAA raises GH release and [Ca2+](i) in cultured caprine anterior pituilary cells, and 2) Ca2+ influx from the medium may be responsible for the cellular action of NEAA.

    Original languageEnglish
    Pages (from-to)99-107
    Number of pages9
    JournalDomestic Animal Endocrinology
    Issue number2
    Publication statusPublished - 1997 Mar 1

    ASJC Scopus subject areas

    • Food Animals
    • Animal Science and Zoology
    • Endocrinology


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