Green tea polyphenol epigallocatechin-3-gallate blocks PDGF-induced proliferation and migration of rat pancreatic stellate cells

Atsushi Masamune, Kazuhiro Kikuta, Masahiro Satoh, Noriaki Suzuki, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Aim: To clarify the effects of epigallocatechin-3-gallate (EGCG) on the platelet-derived growth factor (PDGF)-BB-induced proliferation and migration of pancreatic stellate cells (PSCs). Methods: PSCs were isolated from rat pancreas tissue and used in their culture-activated, myofibroblast-like phenotype. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2′-deoxyuridine. Cell migration was assessed using modified Boyden chambers. Cyclin D1, p21Waf1, and p27Kip1 expression and phosphorylation of PDGF β-receptor, extracellular signal-regulated kinase, and Akt were examined by Western blotting. Activation of phosphatidylinositol 3-kinase was examined by kinase assay using phosphatidylinositol as a substrate. Cell cycle was assessed by flow cytometry after staining with propidium iodide. Results: EGCG at non-cytotoxic concentrations inhibited PDGF-induced proliferation and migration. This effect was associated with the inhibition of cell cycle progression beyond the G1 phase, decreased cyclin D1 and increased p27Kip1 expression. EGCG inhibited tyrosine phosphorylation of PDGF β-receptor and downstream activation of extracellular signal-regulated kinase and phosphatidylinositol 3-kinase/Akt pathways. Conclusion: EGCG inhibited PDGF-BB-induced proliferation and migration of PSCs through the inhibition of PDGF-mediated signaling pathways.

Original languageEnglish
Pages (from-to)3368-3374
Number of pages7
JournalWorld Journal of Gastroenterology
Volume11
Issue number22
DOIs
Publication statusPublished - 2005 Jun 14

Keywords

  • Epigallocatechin-3-gallate
  • Green tea
  • Migration
  • Pancreatic fibrosis
  • Pancreatic stellate cells
  • Pancreatitis
  • Platelet-derived growth factor
  • Proliferation

ASJC Scopus subject areas

  • Gastroenterology

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