Green fluorescent protein-transgenic rat: A tool for organ transplantation research

Yoji Hakamata, Kazunori Tahara, Hiroo Uchida, Yasunaru Sakuma, Masahiko Nakamura, Akihiro Kume, Takashi Murakami, Masafumi Takahashi, Riichi Takahashi, Masumi Hirabayashi, Masatsugu Ueda, Ichiro Miyoshi, Noriyuki Kasai, Eiji Kobayashi

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The purpose of this study is to evaluate green fluorescent protein (GFP) transgenic rats for use as a tool for organ transplantation research. The GFP gene construct was designed to express ubiquitously. By flow cytometry, the cells obtained from the bone marrow, spleen, and peripheral blood of the GFP transgenic rats consisted of 77, 91, and 75% GFP-positive cells, respectively. To examine cell migration of GFP-positive cells after organ transplantation, pancreas graft with or without spleen transplantation, heart graft with or without lung transplantation, auxiliary liver and small bowel transplantation were also performed from GFP transgenic rat to LEW (RT11) rats under a 2-week course of 0.64 mg/kg tacrolimus administration. GFP-positive donor cells were detected in the fully allogenic LEW rats after organ transplantation. These results showed that GFP transgenic rat is a useful tool for organ transplantation research such as cell migration study after organ transplantation without donor cell staining.

Original languageEnglish
Pages (from-to)779-785
Number of pages7
JournalBiochemical and biophysical research communications
Volume286
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Donor cell migration
  • Flow cytometry
  • Green fluorescent protein
  • Transgenic rat
  • Transplantation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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