Grease matrix as a versatile carrier of proteins for serial crystallography

Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi KameshimaTakaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

Original languageEnglish
Pages (from-to)61-63
Number of pages3
JournalNature Methods
Volume12
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Sugahara, M., Mizohata, E., Nango, E., Suzuki, M., Tanaka, T., Masuda, T., Tanaka, R., Shimamura, T., Tanaka, Y., Suno, C., Ihara, K., Pan, D., Kakinouchi, K., Sugiyama, S., Murata, M., Inoue, T., Tono, K., Song, C., Park, J., ... Iwata, S. (2014). Grease matrix as a versatile carrier of proteins for serial crystallography. Nature Methods, 12(1), 61-63. https://doi.org/10.1038/nmeth.3172