Graphene as excellent support for rapid and efficient near infrared-assisted tryptic proteolysis

Chia Feng Chang, Quang Duc Truong, Jiann Ruey Chen

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A rapid and efficient tryptic proteolysis approach has been developed by combining graphene with near infrared (NIR)-assisted protein digestion. Based on the unique properties of graphene such as strong absorption ability of electromagnetic radiation over a wide range of wavelengths and excellent thermal conductivity, we developed a novel fast NIR-assisted tryptic digestion method using graphene, allowing the entire time for identification of protein to be greatly reduced. Bovine serum albumin (BSA), myoglobin (Myo), lysozyme (Lys) and cytochrome c (Cyt-c) were denatured with a digestion time as short as 3. s. Peptide fragments were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Equivalent or better digestion efficiency was observed comparing to current in-solution digestion methods. This novel graphene-based NIR-assisted digestion approach may provide a promising proteomic identification technique to biomedical and clinical research.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume104
DOIs
Publication statusPublished - 2013 Apr 1

Keywords

  • Graphene
  • MALDI-TOF MS
  • Near infrared energy
  • Proteolysis

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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