Abstract
A rapid and efficient tryptic proteolysis approach has been developed by combining graphene with near infrared (NIR)-assisted protein digestion. Based on the unique properties of graphene such as strong absorption ability of electromagnetic radiation over a wide range of wavelengths and excellent thermal conductivity, we developed a novel fast NIR-assisted tryptic digestion method using graphene, allowing the entire time for identification of protein to be greatly reduced. Bovine serum albumin (BSA), myoglobin (Myo), lysozyme (Lys) and cytochrome c (Cyt-c) were denatured with a digestion time as short as 3. s. Peptide fragments were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Equivalent or better digestion efficiency was observed comparing to current in-solution digestion methods. This novel graphene-based NIR-assisted digestion approach may provide a promising proteomic identification technique to biomedical and clinical research.
Original language | English |
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Pages (from-to) | 221-228 |
Number of pages | 8 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 104 |
DOIs | |
Publication status | Published - 2013 Apr 1 |
Keywords
- Graphene
- MALDI-TOF MS
- Near infrared energy
- Proteolysis
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry