Graft linker immobilization for spatial control of protein immobilization inside fused microchips

Kentaro Shirai, Björn Renberg, Kae Sato, Kazuma Mawatari, Tomohiro Konno, Kazuhiko Ishihara, Takehiko Kitamori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fused silica glass microchips have several attractive features for lab-on-a-chip applications; they can be machined with excellent precision down to nanospace; are stable; transparent and can be modified with a range of silanization agents to change channel surface properties. For immobilization, however, ligands must be added after bonding, since the harsh bonding conditions using heat or hydrofluoric acid would remove all prior immobilized ligands. For spatial control over immobilization, UV-mediated immobilization offers several advantages; spots can be created in parallel, the feature size can be made small, and spatial control over patterns and positions is excellent. However, UV sensitive groups are often based on hydrophobic chemical moieties, which unfortunately result in greater non-specific binding of biomolecules, especially proteins. Here, we present techniques in which any -CHx (x51,2,3) containing surface coating can be used as foundation for grafting a hydrophilic linker with a chemical anchor, a carboxyl group, to which proteins and amine containing molecules can be covalently coupled. Hence, the attractive features of many well-known protein and biomolecule repelling polymer coatings can be utilized while achieving site-specific immobilization only to pre-determined areas within the bonded microchips.

Original languageEnglish
Pages (from-to)4251-4255
Number of pages5
JournalElectrophoresis
Volume30
Issue number24
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • Lab-on-a-chip
  • Microchips
  • Protein immobilization
  • UV

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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  • Cite this

    Shirai, K., Renberg, B., Sato, K., Mawatari, K., Konno, T., Ishihara, K., & Kitamori, T. (2009). Graft linker immobilization for spatial control of protein immobilization inside fused microchips. Electrophoresis, 30(24), 4251-4255. https://doi.org/10.1002/elps.200900227