Gradient hydrogels for high throughput drug screening

S. Ostrovidov, N. Annabi, F. Dehghani, A. Seidi, M. Ramalingam, H. Kaji, A. Khademhosseini

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study we demonstrate that hydrogels loaded with a concentration gradient of an active molecule can be used as analytical tools for high throughput screening (HTS) and drug discovery applications. We used a microfluidic mixer to fabricate poly(ethylene glycol) (PEG) hydrogels that contained chemical concentration gradients. Each hydrogel was placed in culture medium in the vicinity of preosteoblast MC3T3-E1 cell layers. The controlled release of the toxin from the hydrogel in a gradient manner was used to study the effect of various chemical concentrations on cell viability. Given its simplicity, ease of storage and portability, this platform is a potentially useful approach to screen a range of drug concentrations simultaneously.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1463-1465
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Gradient
  • Hydrogel
  • Microfluidic
  • Toxin

ASJC Scopus subject areas

  • Control and Systems Engineering

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