Abstract
The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.
| Original language | English |
|---|---|
| Pages (from-to) | 750-755 |
| Number of pages | 6 |
| Journal | Biochemical and biophysical research communications |
| Volume | 391 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Jan 1 |
| Externally published | Yes |
Keywords
- 3′,6′-IsoLD1
- 3′-IsoLM1
- Knockout mice
- Lacto-series ganglioside
- Lc3-synthase
- Monoclonal antibody
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Cite this
Kato, Y., Kuan, C. T., Chang, J., Kaneko, M. K., Ayriss, J., Piao, H., Chandramohan, V., Pegram, C., McLendon, R. E., Fredman, P., Månsson, J. E., & Bigner, D. D. (2010). GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice. Biochemical and biophysical research communications, 391(1), 750-755. https://doi.org/10.1016/j.bbrc.2009.11.132