Abstract
The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.
Original language | English |
---|---|
Pages (from-to) | 750-755 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 391 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2010 Jan 1 |
Externally published | Yes |
Keywords
- 3′,6′-IsoLD1
- 3′-IsoLM1
- Knockout mice
- Lacto-series ganglioside
- Lc3-synthase
- Monoclonal antibody
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology