GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice

Yukinari Kato; Chien Tsun Kuan; Jinli Chang; Mika Kato Kaneko; Joanne Ayriss; Hailan Piao; Vidyalakshmi Chandramohan; Charles Pegram; Roger E. McLendon; Pam Fredman; Jan Eric Månsson; Darell D. Bigner

doi:10.1016/j.bbrc.2009.11.132

GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice

Yukinari Kato, Chien Tsun Kuan, Jinli Chang, Mika Kato Kaneko, Joanne Ayriss, Hailan Piao, Vidyalakshmi Chandramohan, Charles Pegram, Roger E. McLendon, Pam Fredman, Jan Eric Månsson, Darell D. Bigner

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG₃ subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.

Original language	English
Pages (from-to)	750-755
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.132
Publication status	Published - 2010 Jan 1
Externally published	Yes

Keywords

3′,6′-IsoLD1
3′-IsoLM1
Knockout mice
Lacto-series ganglioside
Lc3-synthase
Monoclonal antibody

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kato, Y., Kuan, C. T., Chang, J., Kaneko, M. K., Ayriss, J., Piao, H., Chandramohan, V., Pegram, C., McLendon, R. E., Fredman, P., Månsson, J. E., & Bigner, D. D. (2010). GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice. Biochemical and biophysical research communications, 391(1), 750-755. https://doi.org/10.1016/j.bbrc.2009.11.132

GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice. / Kato, Yukinari; Kuan, Chien Tsun; Chang, Jinli; Kaneko, Mika Kato; Ayriss, Joanne; Piao, Hailan; Chandramohan, Vidyalakshmi; Pegram, Charles; McLendon, Roger E.; Fredman, Pam; Månsson, Jan Eric; Bigner, Darell D.

In: Biochemical and biophysical research communications, Vol. 391, No. 1, 01.01.2010, p. 750-755.

Research output: Contribution to journal › Article › peer-review

Kato, Y, Kuan, CT, Chang, J, Kaneko, MK, Ayriss, J, Piao, H, Chandramohan, V, Pegram, C, McLendon, RE, Fredman, P, Månsson, JE & Bigner, DD 2010, 'GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice', Biochemical and biophysical research communications, vol. 391, no. 1, pp. 750-755. https://doi.org/10.1016/j.bbrc.2009.11.132

Kato, Yukinari ; Kuan, Chien Tsun ; Chang, Jinli ; Kaneko, Mika Kato ; Ayriss, Joanne ; Piao, Hailan ; Chandramohan, Vidyalakshmi ; Pegram, Charles ; McLendon, Roger E. ; Fredman, Pam ; Månsson, Jan Eric ; Bigner, Darell D. / GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice. In: Biochemical and biophysical research communications. 2010 ; Vol. 391, No. 1. pp. 750-755.

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title = "GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice",

abstract = "The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.",

keywords = "3′,6′-IsoLD1, 3′-IsoLM1, Knockout mice, Lacto-series ganglioside, Lc3-synthase, Monoclonal antibody",

author = "Yukinari Kato and Kuan, {Chien Tsun} and Jinli Chang and Kaneko, {Mika Kato} and Joanne Ayriss and Hailan Piao and Vidyalakshmi Chandramohan and Charles Pegram and McLendon, {Roger E.} and Pam Fredman and M{\aa}nsson, {Jan Eric} and Bigner, {Darell D.}",

note = "Funding Information: We thank Mr. Ling Wang of Duke University Medical Center and Mrs. Birgitta Dellheden of University of Gothenburg for immunohistochemical analysis and ganglioside isolation, respectively. We also thank Dr. Chifumi Kitanaka of Yamagata University School of Medicine for his helpful suggestion and Ms. Janet Parsons of Duke University Medical Center for editing the manuscript. This study was supported by NIH Grants 5P50 CA108786 , 5P50 NS20023 , and R37 CA011898 . Y.K. was supported by the Global COE Program for Medical Sciences, Japan Society for the Promotion of Science. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.bbrc.2009.11.132",

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AU - Kato, Yukinari

AU - Kuan, Chien Tsun

AU - Chang, Jinli

AU - Kaneko, Mika Kato

AU - Ayriss, Joanne

AU - Piao, Hailan

AU - Chandramohan, Vidyalakshmi

AU - Pegram, Charles

AU - McLendon, Roger E.

AU - Fredman, Pam

AU - Månsson, Jan Eric

AU - Bigner, Darell D.

N1 - Funding Information: We thank Mr. Ling Wang of Duke University Medical Center and Mrs. Birgitta Dellheden of University of Gothenburg for immunohistochemical analysis and ganglioside isolation, respectively. We also thank Dr. Chifumi Kitanaka of Yamagata University School of Medicine for his helpful suggestion and Ms. Janet Parsons of Duke University Medical Center for editing the manuscript. This study was supported by NIH Grants 5P50 CA108786 , 5P50 NS20023 , and R37 CA011898 . Y.K. was supported by the Global COE Program for Medical Sciences, Japan Society for the Promotion of Science. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.

AB - The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.

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KW - 3′-IsoLM1

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KW - Lacto-series ganglioside

KW - Lc3-synthase

KW - Monoclonal antibody

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GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice

Abstract

Keywords

ASJC Scopus subject areas

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