Glyoxalase I overexpression ameliorates renal ischemia-reperfusion injury in rats

Takanori Kumagai, Masaomi Nangaku, Ichiro Kojima, Ryoji Nagai, Julie R. Ingelfinger, Toshio Miyata, Toshiro Fujita, Reiko Inagi

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71 Citations (Scopus)

Abstract

Methylglyoxal (MG), a highly reactive carbonyl compound generated by carbohydrate oxidation and glycolysis, is the major precursor of protein glycation and induces cytotoxicity leading to apoptosis. Although recent studies have emphasized that MG accumulates in not only chronic oxidative stress-related diseases but also acute hypoxic conditions, the pathogenic contribution of MG in acute diseases is unclear. MG is efficiently metabolized by the glyoxalase system, namely, glyoxalase I. We investigated the pathophysiological role of glyoxalase I as an MG detoxifier in rat renal ischemia-reperfusion (I/R) injury. I/R-induced tubulointerstitial injury was associated with a deterioration in renal glyoxalase I activity independent of its cofactor, GSH, as well as an increase in renal MG level. In in vitro studies, knockdown of glyoxalase I by small interference RNA transfection in rat tubular cells exacerbated cell death by hypoxia-reoxygenation compared with control cells. We also examined whether glyoxalase I overexpression prevented renal I/R damage in rats overexpressing human glyoxalase I with enzyme activity in the kidney 17-fold higher than in wild-type. The histological and functional manifestations of I/R in these rats were significantly ameliorated in association with a decrease in intracellular MG adduct accumulation, oxidative stress, and tubular cell apoptosis. In conclusion, glyoxalase I exerts renoprotective effects in renal I/R injury via a reduction in MG accumulation in tubular cells.

Original languageEnglish
Pages (from-to)F912-F921
JournalAmerican Journal of Physiology - Renal Physiology
Volume296
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1

Keywords

  • Acute renal failure
  • Advanced glycation end products
  • Methylglyoxal
  • Oxidative stress
  • Tubular cells

ASJC Scopus subject areas

  • Physiology
  • Urology

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    Kumagai, T., Nangaku, M., Kojima, I., Nagai, R., Ingelfinger, J. R., Miyata, T., Fujita, T., & Inagi, R. (2009). Glyoxalase I overexpression ameliorates renal ischemia-reperfusion injury in rats. American Journal of Physiology - Renal Physiology, 296(4), F912-F921. https://doi.org/10.1152/ajprenal.90575.2008