Glycosylation of the alpha and beta tubulin by sialyloligosaccharides.

Mizuki Hino, Isao Kijima-Suda, Yoshitaka Nagai, Hiroshi Hosoya

Research output: Contribution to journalArticlepeer-review

Abstract

To examine whether alpha and beta tubulin are glycoproteins, we used a pyridylamino labeling method and a monoclonal antibody, SG3-1, raised against NeuAcalpha2-3Gal structure. Alpha and beta tubulin from both pig brain and HeLa cells were positive for the SG3-1 antibody by immunoblot assay. Sialidase treatment reduced the reactivity of the SG3-1 antibody to alpha and beta tubulin molecules. N-linked oligosaccharide analysis also showed that alpha and beta tubulin are glycosylated. Moreover, immunofluorescence analysis showed that the filamentous structure recognized by the SG3-1 antibody was overlapped with microtubules, especially in the vicinity of the nucleus. These results indicate that alpha and beta tubulin are glycosylated with sialyloligosaccharides.

Original languageEnglish
Pages (from-to)709-715
Number of pages7
JournalZoological Science
Volume20
Issue number6
DOIs
Publication statusPublished - 2003 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology

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