Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus

Y. Kariya; S. Watanabe; Y. Ochiai; K. Murata

doi:10.1016/0305-0491(90)90092-8

Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus

Y. Kariya, S. Watanabe, Y. Ochiai, K. Murata

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10^-3 mol SO₄^2-, 1.51 × 10^-3 mol N-acetylgalactosamine and 0.98 × 10³ mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl₂ and MgCl₂. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl₂ and MgCl₂ increased.

Original language	English
Pages (from-to)	387-392
Number of pages	6
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(90)90092-8
Publication status	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

Access to Document

10.1016/0305-0491(90)90092-8

Fingerprint Dive into the research topics of 'Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus'. Together they form a unique fingerprint.

Cite this

@article{22d354495d7e40c09fc053724be237df,

title = "Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus",

abstract = "1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.",

author = "Y. Kariya and S. Watanabe and Y. Ochiai and K. Murata",

year = "1990",

doi = "10.1016/0305-0491(90)90092-8",

language = "English",

volume = "95",

pages = "387--392",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

number = "2",

}

TY - JOUR

T1 - Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus

AU - Kariya, Y.

AU - Watanabe, S.

AU - Ochiai, Y.

AU - Murata, K.

PY - 1990

Y1 - 1990

N2 - 1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.

AB - 1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.

UR - http://www.scopus.com/inward/record.url?scp=0025169818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025169818&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(90)90092-8

DO - 10.1016/0305-0491(90)90092-8

M3 - Article

AN - SCOPUS:0025169818

VL - 95

SP - 387

EP - 392

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

SN - 1096-4959

IS - 2

ER -