TY - JOUR
T1 - Glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus
AU - Kariya, Y.
AU - Watanabe, S.
AU - Ochiai, Y.
AU - Murata, K.
N1 - Funding Information:
Acknowledgements--The expenses of the present study were covered in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan. The authors express their sincere thanks to Professor N. Seno, Ochano-mizu Women's University, for her kind gift of Ch-SE and invaluable suggestions, and to Dr. Y. Yokoyama, Department of Medicine and Physical Therapy, School of Medicine, The University of Tokyo, for her helpful technical instruction. Thanks are also due to Dr. K. Yoshida and Dr. A. Tawada, Seikagaku Kogyo Co., for IR spectrophotometry. We also express heartfelt thanks to Professor K. Tanaka, Faculty of Dentistry, Nagasaki University, and Professor R. Hayakawa, Department of Applied Physics, Faculty of Engineering, The University of Tokyo, for their advice on molecular configuration analyses.
PY - 1990
Y1 - 1990
N2 - 1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.
AB - 1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.
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U2 - 10.1016/0305-0491(90)90092-8
DO - 10.1016/0305-0491(90)90092-8
M3 - Article
AN - SCOPUS:0025169818
VL - 95
SP - 387
EP - 392
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 2
ER -