1. 1. Glycosaminoglycan (GAG) was isolated from the body wall of sea cucumber Stichopus japonicus. 2. 2. One gram of GAG contained 3.12 × 10-3 mol SO42-, 1.51 × 10-3 mol N-acetylgalactosamine and 0.98 × 103 mol glucuronic acid, suggesting it to be a chondroitin sulfate. 3. 3. Ultracentrifugal analyses of the GAG revealed that the molecular weight and sedimentation coefficient were 18,000 and 1.6 S, respectively, in distilled water. 4. 4. These values were increased in the presence of 0.1 M each of NaCl, CaCl2 and MgCl2. 5. 5. The sedimentation coefficient was increased when the concentration of NaCl was increased, whereas it was decreased when those of CaCl2 and MgCl2 increased.
|Number of pages||6|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - 1990|
ASJC Scopus subject areas
- Molecular Biology