Abstract
Two glycan isoforms of transferrin (Tf), Tf-1 and Tf-2, were found in cerebrospinal fluid (CSF). Tf-1 concentration in CSF was reduced in idiopathic normal pressure hydrocephalus (iNPH), an elderly dementia caused by abnormal metabolism of CSF, whereas Tf-2 concentration was not. The reduction of Tf-1 concentration was not found in Alzheimer’s disease, indicating that Tf-1 can distinguish iNPH from Alzheimer’s disease. Glycan analysis revealed that Tf-1 has unique biantennary asialo- and agalacto-complex N-glycans, which carry bisecting β1,4-GlcNAc and core α1,6-fucose. In contrast, Tf-2 has α2,6-sialylglycan like serum Tf, suggesting that Tf-2 is derived from serum. Tf-1, a glycoform unique to CSF, is speculated to be produced in brain tissue. Immunohistochemistry using anti-Tf antibody on brain tissues revealed that the choroid plexus, which produces CSF, was strongly stained by anti-Tf antibody. These results suggest that Tf-1 is secreted from the choroid plexus and its secretion is reduced by abnormal metabolism of CSF in iNPH.
Original language | English |
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Title of host publication | Glycoscience |
Subtitle of host publication | Biology and Medicine |
Publisher | Springer Japan |
Pages | 1289-1295 |
Number of pages | 7 |
ISBN (Electronic) | 9784431548416 |
ISBN (Print) | 9784431548409 |
DOIs | |
Publication status | Published - 2015 Jan 1 |
Keywords
- Biomarker
- Cerebrospinal fluid
- Choroid plexus
- Dementia
- Glycan isoforms
- Idiopathic normal pressure hydrocephalus
- Transferrin
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)