Glycan Marker for Idiopathic Normal Pressure Hydrocephalus

Kyoka Hoshi, Yuka Matsumoto, Toshie Saito, Hiromi Ito, Yoshinobu Kariya, Yoshiki Yamaguchi, Katsutoshi Furukawa, Hiroyuki Arai, Masakazu Miyajima, Hajime Arai, Hisashi Narimatsu, Kiyoshi Saito, Takashi Honda, Yasuhiro Hashimoto

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Two glycan isoforms of transferrin (Tf), Tf-1 and Tf-2, were found in cerebrospinal fluid (CSF). Tf-1 concentration in CSF was reduced in idiopathic normal pressure hydrocephalus (iNPH), an elderly dementia caused by abnormal metabolism of CSF, whereas Tf-2 concentration was not. The reduction of Tf-1 concentration was not found in Alzheimer’s disease, indicating that Tf-1 can distinguish iNPH from Alzheimer’s disease. Glycan analysis revealed that Tf-1 has unique biantennary asialo- and agalacto-complex N-glycans, which carry bisecting β1,4-GlcNAc and core α1,6-fucose. In contrast, Tf-2 has α2,6-sialylglycan like serum Tf, suggesting that Tf-2 is derived from serum. Tf-1, a glycoform unique to CSF, is speculated to be produced in brain tissue. Immunohistochemistry using anti-Tf antibody on brain tissues revealed that the choroid plexus, which produces CSF, was strongly stained by anti-Tf antibody. These results suggest that Tf-1 is secreted from the choroid plexus and its secretion is reduced by abnormal metabolism of CSF in iNPH.

Original languageEnglish
Title of host publicationGlycoscience
Subtitle of host publicationBiology and Medicine
PublisherSpringer Japan
Pages1289-1295
Number of pages7
ISBN (Electronic)9784431548416
ISBN (Print)9784431548409
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • Biomarker
  • Cerebrospinal fluid
  • Choroid plexus
  • Dementia
  • Glycan isoforms
  • Idiopathic normal pressure hydrocephalus
  • Transferrin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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