TY - JOUR
T1 - Glucose transporter 4 (GLUT4) mRNA abundance in the adipose tissue and skeletal-muscle tissue of ovariectomized rats treated with 17β-estradiol or progesterone
AU - Sugaya, Ayumi
AU - Sugiyama, Takashi
AU - Yanase, Sachiko
AU - Terada, Yumiko
AU - Toyoda, Nagayasu
PY - 1999/2
Y1 - 1999/2
N2 - Objective: To investigate the effects of 17β-estradiol and progesterone on the expression of glucose transporter 4 (GLUT4) in the adipose tissue and skeletal-muscle tissue of ovariectomized rats. Methods: Female Sprague-Dawley rats (n = 63) received a daily subcutaneous injection of 10 μg, 50 μg, or 250 μg of 17β-estradiol (Group E) or of 1 mg, 5 mg or 25 mg of progesterone (Group P) for 3 days, 7 days, or 10 days (n = 3, at each dose). The expression of GLUT4 mRNA was assessed by performing ribonuclease protection assays. Results: The levels of GLUT4 mRNA in adipose tissue was significantly reduced by treatment with estradiol, 50 μg or 250 μg, relative to findings in control rats (p < 0.01). No such reductions were seen regarding the progesterone treatment. The level of GLUT4 mRNA in skeletal-muscle tissue did not change, regardless of treatment. Conclusion: In ovariectomized rats, higher than physiologic dosages of 17β-estradiol can suppress the expression of GLUT4 mRNA in adipose tissue.
AB - Objective: To investigate the effects of 17β-estradiol and progesterone on the expression of glucose transporter 4 (GLUT4) in the adipose tissue and skeletal-muscle tissue of ovariectomized rats. Methods: Female Sprague-Dawley rats (n = 63) received a daily subcutaneous injection of 10 μg, 50 μg, or 250 μg of 17β-estradiol (Group E) or of 1 mg, 5 mg or 25 mg of progesterone (Group P) for 3 days, 7 days, or 10 days (n = 3, at each dose). The expression of GLUT4 mRNA was assessed by performing ribonuclease protection assays. Results: The levels of GLUT4 mRNA in adipose tissue was significantly reduced by treatment with estradiol, 50 μg or 250 μg, relative to findings in control rats (p < 0.01). No such reductions were seen regarding the progesterone treatment. The level of GLUT4 mRNA in skeletal-muscle tissue did not change, regardless of treatment. Conclusion: In ovariectomized rats, higher than physiologic dosages of 17β-estradiol can suppress the expression of GLUT4 mRNA in adipose tissue.
KW - 17β-estradiol
KW - Glucose transporter 4 (GLUT4)
KW - Progesterone
KW - Rat
KW - mRNA
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U2 - 10.1111/j.1447-0756.1999.tb01115.x
DO - 10.1111/j.1447-0756.1999.tb01115.x
M3 - Article
C2 - 10067007
AN - SCOPUS:0032984555
VL - 25
SP - 9
EP - 14
JO - Journal of Obstetrics and Gynaecology Research
JF - Journal of Obstetrics and Gynaecology Research
SN - 1341-8076
IS - 1
ER -