Glucose transporter 4 (GLUT4) mRNA abundance in the adipose tissue and skeletal-muscle tissue of ovariectomized rats treated with 17β-estradiol or progesterone

Ayumi Sugaya, Takashi Sugiyama, Sachiko Yanase, Yumiko Terada, Nagayasu Toyoda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Objective: To investigate the effects of 17β-estradiol and progesterone on the expression of glucose transporter 4 (GLUT4) in the adipose tissue and skeletal-muscle tissue of ovariectomized rats. Methods: Female Sprague-Dawley rats (n = 63) received a daily subcutaneous injection of 10 μg, 50 μg, or 250 μg of 17β-estradiol (Group E) or of 1 mg, 5 mg or 25 mg of progesterone (Group P) for 3 days, 7 days, or 10 days (n = 3, at each dose). The expression of GLUT4 mRNA was assessed by performing ribonuclease protection assays. Results: The levels of GLUT4 mRNA in adipose tissue was significantly reduced by treatment with estradiol, 50 μg or 250 μg, relative to findings in control rats (p < 0.01). No such reductions were seen regarding the progesterone treatment. The level of GLUT4 mRNA in skeletal-muscle tissue did not change, regardless of treatment. Conclusion: In ovariectomized rats, higher than physiologic dosages of 17β-estradiol can suppress the expression of GLUT4 mRNA in adipose tissue.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalJournal of Obstetrics and Gynaecology Research
Volume25
Issue number1
DOIs
Publication statusPublished - 1999 Feb

Keywords

  • 17β-estradiol
  • Glucose transporter 4 (GLUT4)
  • Progesterone
  • Rat
  • mRNA

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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