Glucose sensor using a phospholipid polymer-based enzyme immobilization method

Hiroyuki Kudo, Tamon Yagi, Ming Xing Chu, Hirokazu Saito, Nobuyuki Morimoto, Yasuhiko Iwasaki, Kazunari Akiyoshi, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

An electroenzymatic glucose sensor based on a simple enzyme immobilization technique was constructed and tested. The glucose sensor measures glucose concentrations as changes of oxygen concentrations induced by enzymatic reactions. The immobilizing procedure was developed with the purpose of producing wearable biosensors for clinical use. Two types of biocompatible polymers, 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with dodecyl methacrylate (PMD) and MPC copolymerized with 2-ethylhexyl methacrylat, were compared as a sensitive membrane of biosensors. The PMD enzyme membrane had a better response time. Linearity, reproducibility, effect of the concentrations of immobilized enzyme and drifts of sensor characteristics in long-term tests were also investigated. The linear characteristics were confirmed with glucose concentration from 0.01 to 2.00 mmol/l, with a coefficient of determination of 0.9999. The average output current for 1 mmol/l and the standard deviation were 0.992 and 0.0283 μA. Significant changes in the sensor's characteristics were not observed for 2 weeks when it was kept in a refrigerator at 4 °C. Because of the simple procedure, the enzyme immobilization method is not only useful for wearable devices but also other devices such as micro total analysis systems.

Original languageEnglish
Pages (from-to)1269-1274
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number4
DOIs
Publication statusPublished - 2008 Jun 1
Externally publishedYes

Keywords

  • Biosensor
  • Electrochemical
  • Electroenzymatic
  • Enzyme immobilization
  • Glucose sensor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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