Glucose-induced expression of MIP-1 genes requires O-GlcNAc transferase in monocytes

Toshihiro Chikanishi, Ryoji Fujiki, Waka Hashiba, Hiroki Sekine, Atsushi Yokoyama, Shigeaki Kato

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

O-glycosylation has emerged as an important modification of nuclear proteins, and it appears to be involved in gene regulation. Recently, we have shown that one of the histone methyl transferases (MLL5) is activated through O-glycosylation by O-GlcNAc transferase (OGT). Addition of this monosaccharide is essential for forming a functional complex. However, in spite of the abundance of OGT in the nucleus, the impact of nuclear O-glycosylation by OGT remains largely unclear. To address this issue, the present study was undertaken to test the impact of nuclear O-glycosylation in a monocytic cell line, THP-1. Using a cytokine array, MIP-1α and -1β genes were found to be regulated by nuclear O-glycosylation. Biochemical purification of the OGT interactants from THP-1 revealed that OGT is an associating partner for distinct co-regulatory complexes. OGT recruitment and protein O-glycosylation were observed at the MIP-1α gene promoter; however, the known OGT partner (HCF-1) was absent when the MIP-1α gene promoter was not activated. From these findings, we suggest that OGT could be a co-regulatory subunit shared by functionally distinct complexes supporting epigenetic regulation.

Original languageEnglish
Pages (from-to)865-870
Number of pages6
JournalBiochemical and biophysical research communications
Volume394
Issue number4
DOIs
Publication statusPublished - 2010 Apr 16

Keywords

  • MIP-1α
  • Nuclear O-glycosylation
  • O-GlcNAc transferase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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