Glucose degradation product methylglyoxal enhances the production of vascular endothelial growth factor in peritoneal cells: Role in the functional and morphological alterations of peritoneal membranes in peritoneal dialysis

Reiko Inagi, Toshio Miyata, Takashi Yamamoto, Daisuke Suzuki, Ken Ichi Urakami, Akira Saito, Charles Van Ypersele De Strihou, Kiyoshi Kurokawa

Research output: Contribution to journalArticlepeer-review

158 Citations (Scopus)

Abstract

Peritoneal membrane permeability deteriorates in peritoneal dialysis (PD) patients. We test whether glucose degradation products (GDPs) in PD fluids, glyoxal, methylglyoxal and 3-deoxyglucosone, stimulate the production of vascular endothelial growth factor (VEGF), a factor known to enhance vascular permeability and angiogenesis. VEGF increased in cultured rat mesothelial and human endothelial cells exposed to methylglyoxal, but not to glyoxal or 3-deoxyglucosone. VEGF also increased in peritoneal tissue of rats given intraperitoneally methylglyoxal. VEGF and carboxymethyllysine (CML) (formed from GDPs) co-localized immunohistochemically in mesothelial layer and vascular walls of the peritoneal membrane of patients given chronic PD. By contrast, in the peritoneum of non-uremic subjects, VEGF was identified only in vascular walls, in the absence of CML. VEGF production induced by GDPs may play a role in the progressive deterioration of the peritoneal membrane.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalFEBS Letters
Volume463
Issue number3
DOIs
Publication statusPublished - 1999 Dec 17

Keywords

  • Glucose degradation product
  • Methylglyoxal
  • Peritoneal dialysis
  • Peritoneal membrane
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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