Glucose and lactate biosensors prepared by a layer-by-layer deposition of concanavalin A and mannose-labeled enzymes: Electrochemical response in the presence of electron mediators

Y. Kobayashi, T. Hoshi, J. I. Anzai

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

An electrochemical response of glucose and lactate biosensors which were prepared by coating a platinum electrode with a thin film composed of concanavalin A and mannose-labeled glucose oxidase (GOx) or lactate oxidase (LOx) was evaluated in the presence of ferrocene derivatives as electron mediator. Both glucose and lactate biosensors showed catalytic current to glucose and lactate, respectively, in cyclic voltammetry, suggesting that the ferrocene derivatives can mediate electron transport smoothly from the reduced forms of GOx and LOx in the thin films to the electrode. Among the three kinds of ferrocene derivatives used, ferrocenylmethanol was found to be the most suitable electron mediator because of its low oxidation potential. The glucose and lactate sensors gave useful calibration graphs, in which higher detection limits were reached as compared with those observed when the sensors were operated in the absence of electron mediator.

Original languageEnglish
Pages (from-to)755-757
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume49
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • Biosensor
  • Electron mediator
  • Enzyme multilayer film

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Fingerprint Dive into the research topics of 'Glucose and lactate biosensors prepared by a layer-by-layer deposition of concanavalin A and mannose-labeled enzymes: Electrochemical response in the presence of electron mediators'. Together they form a unique fingerprint.

Cite this