Glucocorticoid inhibits expression of V-1, a catecholamine biosynthesis regulatory protein, in cultured adrenal medullary cells

Yusuke Hiwatashi, Yusuke Kurahashi, Risa Hatada, Susumu Ueno, Takao Honma, Nobuyuki Yanagihara, Haruko Yanase, Toshihiko Iwanaga, Yasushi Ohizumi, Tohru Yamakuni

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

V-1 acts as a positive and coordinate regulator of gene expression of catecholamine biosynthetic enzymes in PC12D cells. The present study was conducted to investigate the mechanism controlling expression of V-1 in the adrenal gland. Immunohistochemical analysis demonstrated that noradrenergic chromaffin cells more highly expressed V-1 than adrenergic chromaffin cells preferentially expressing the glucocorticoid receptor in rat adrenal glands. Western blotting showed that in cultured bovine adrenal medullary cells, dexamethasone, a synthetic glucocorticoid, inhibited expression of V-1, and that this inhibition was prevented by RU-486, a glucocorticoid receptor antagonist. These results suggest that adrenal expression of V-1 is differentially controlled by glucocorticoids through the specific receptor, and that thereby V-1 regulates catecholamine biosynthesis in a catecholaminergic phenotype-dependent manner.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalFEBS Letters
Volume528
Issue number1-3
DOIs
Publication statusPublished - 2002 Sep 25

Keywords

  • Adrenal chromaffin cells
  • Catecholamine biosynthesis
  • Glucocorticoid
  • V-1 protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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