Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis

Taika Muto, Hirokazu Taniguchi, Ryoji Kushima, Hitoshi Tsuda, Hirotaka Yonemori, Chen Chen, Yutaka Sugihara, Kano Sakamoto, Yukiko Kobori, Helen Palmer, Yukiko Nakamura, Takeshi Tomonaga, Hiroshi Tanaka, Hiroshi Mizushima, Shin Fujita, Tadashi Kondo

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Colorectal cancer is one of the leading causes of cancer death worldwide. To identify candidates for biomarkers and therapeutic targets, we investigated the proteome of colorectal cancer tissues. Using 2D-DIGE in combination with our original large format electrophoresis apparatus, we compared surgically resected normal and tumor tissues from 53 patients with colorectal cancer. We focused on proteins with an alkaline p. I using IPG gels for the alkaline range. We observed 1687 protein spots, and found 100 spots with statistical (p < 0.01) and significant (>2-fold) differences between the normal and the tumor tissue groups. Among these 100 protein spots, five showed a different intensity between tumor tissues from the stage-II and the stage-III patients. MS experiments revealed that these 100 protein spots corresponded to 58 unique proteins. These included six proteins which had not been previously reported to be associated with colorectal cancer. Among these proteins, five were not reported in any type of malignancy. IEF/western blotting confirmed the differences in protein expression between the normal and the tumor tissues. These results may provide an insight for biomarker development and drug target discovery in colorectal cancer.

Original languageEnglish
Pages (from-to)858-873
Number of pages16
JournalJournal of Proteomics
Volume74
Issue number6
DOIs
Publication statusPublished - 2011 May 16

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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