Ginsenoside Rg1-induced antidepressant effects involve the protection of astrocyte gap junctions within the prefrontal cortex

Can Jin, Zhen Zhen Wang, Heng Zhou, Yu Xia Lou, Jiao Chen, Wei Zuo, Man Tong Tian, Zhi Qi Wang, Guo Hua Du, Ichiro Kawahata, Tohru Yamakuni, Yi Zhang, Nai Hong Chen, Dan Shen Zhang

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Ginsenoside Rg1 (Rg1) exhibits antidepressant-like activity by increasing neurogenesis and dendritic spine density without discernible side effects. However, the molecular mechanisms underlying Rg1 antidepressant activity remain poorly understood. As the dysfunction of gap junctions between astrocytes in the prefrontal cortex (PFC) is implicated in major depression disorder, the aim of this study was to investigate the effects of Rg1 on astrocyte gap junctions in the PFC. Rats exposed to chronic unpredictable stress (CUS) were administered Rg1 (5, 10, and 20 mg/kg) for 28 days and analyzed for depressive symptoms using the sucrose preference and forced swimming tests. Functional and morphological changes of gap junction channels in the PFC were evaluated using dye transfer and electron microscopy, respectively. The expression of connexin 43 (Cx43) was analyzed by western blotting. Rg1 markedly alleviated depression-like behavior in rats. Long-term Rg1 treatment of CUS-exposed rats also significantly prevented the decrease in dye diffusion and improved the ultrastructure of astrocyte gap junctions in the PFC, indicating beneficial effects on the functional activity of gap junction channels in the brain. In addition, Rg1 upregulated Cx43 expression in the PFC reduced by CUS exposure, which significantly correlated with its antidepressant-like effects. The results demonstrate that Rg1-induced antidepressant effects are might be mediated, in part, by protecting astrocyte gap junctions within the prefrontal cortex.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
Volume75
DOIs
Publication statusPublished - 2017 Apr 3

Keywords

  • Chronic unpredictable stress
  • Connexin 43
  • Depression
  • Gap junction
  • Ginsenoside Rg1

ASJC Scopus subject areas

  • Pharmacology
  • Biological Psychiatry

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