Gibberellin regulates pollen viability and pollen tube growth in rice

Tory Chhun, Koichiro Aya, Kenji Asano, Eiji Yamamoto, Yoichi Morinaka, Masao Watanabe, Hidemi Kitano, Motoyuki Ashikari, Makoto Matsuoka, Miyako Ueguchi-Tanaka

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)


Gibberellins (GAs) play many biological roles in higher plants. We collected and performed genetic analysis on rice (Oryza sativa) GA-related mutants, including GA-deficient and GA-insensitive mutants. Genetic analysis of the mutants revealed that rice GA-deficient mutations are not transmitted as Mendelian traits to the next generation following self-pollination of F1 heterozygous plants, although GA-insensitive mutations are transmitted normally. To understand these differences in transmission, we examined the effect of GA on microsporogenesis and pollen tube elongation in rice using new GA-deficient and GA-insensitive mutants that produce semifertile flowers. Phenotypic analysis revealed that the GA-deficient mutant reduced pollen elongation1 is defective in pollen tube elongation, resulting in a low fertilization frequency, whereas the GA-insensitive semidominant mutant Slr1-d3 is mainly defective in viable pollen production. Quantitative RT-PCR revealed that GA biosynthesis genes tested whose mutations are transmitted to the next generation at a lower frequency are preferentially expressed after meiosis during pollen development, but expression is absent or very low before the meiosis stage, whereas GA signal-related genes are actively expressed before meiosis. Based on these observations, we predict that the transmission of GA-signaling genes occurs in a sporophytic manner, since the protein products and/or mRNA transcripts of these genes may be introduced into pollen-carrying mutant alleles, whereas GA synthesis genes are transmitted in a gametophytic manner, since these genes are preferentially expressed after meiosis.

Original languageEnglish
Pages (from-to)3876-3888
Number of pages13
JournalPlant Cell
Issue number12
Publication statusPublished - 2007 Dec

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology


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