Gibberellin biosynthesis and response during Arabidopsis seed germination

Mikihiro Ogawa, Atsushi Hanada, Yukika Yamauchi, Ayuko Kuwahara, Yuji Kamiya, Shinjiro Yamaguchi

    Research output: Contribution to journalArticlepeer-review

    627 Citations (Scopus)

    Abstract

    The hormone-mediated control of plant growth and development involves both synthesis and response. Previous studies have shown that gibberellin (GA) plays an essential role in Arabidopsis seed germination. To learn how GA stimulates seed germination, we performed comprehensive analyses of GA biosynthesis and response using gas chromatography-mass spectrometry and oligonucleotide-based DNA microarray analysis. In addition, spatial correlations between GA biosynthesis and response were assessed by in situ hybridization. We identified a number of transcripts, the abundance of which is modulated upon exposure to exogenous GA. A subset of these GA-regulated genes was expressed in accordance with an increase in endogenous active GA levels, which occurs just before radicle emergence. The GA-responsive genes identified include those responsible for synthesis, transport, and signaling of other hormones, suggesting the presence of uncharacterized crosstalk between GA and other hormones. In situ hybridization analysis demonstrated that the expression of GA-responsive genes is not restricted to the predicted site of GA biosynthesis, suggesting that GA itself, or GA signals, is transmitted across different cell types during Arabidopsis seed germination.

    Original languageEnglish
    Pages (from-to)1591-1604
    Number of pages14
    JournalPlant Cell
    Volume15
    Issue number7
    DOIs
    Publication statusPublished - 2003 Jul 1

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology

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