Giant liposome as a biochemical reactor: Transcription of DNA and transportation by laser tweezers

Kanta Tsumoto, Shin Ichirou M. Nomura, Yoichi Nakatani, Kenichi Yoshikawa

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Transcription of T7 DNA has been performed within cell-sized (∼10 μm) liposomes that were formed by natural hydration of phospholipid films. From direct observation by fluorescence microscopy, it was confirmed that the cell-sized liposome behaves as a barrier that can prevent the attack of RNase in the bathing solution surrounding it. It is shown that the liposome encapsulating the transcripts can be transported by use of laser tweezers. Such newly developed procedures on cell-sized liposomes would have applications in the microlaboratory of biochemistry and molecular biology.

Original languageEnglish
Pages (from-to)7225-7228
Number of pages4
JournalLangmuir
Volume17
Issue number23
DOIs
Publication statusPublished - 2001 Nov 13
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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