Giant Acceleration of Diffusion Observed in a Single-Molecule Experiment on F1-ATPase

Ryunosuke Hayashi, Kazuo Sasaki, Shuichi Nakamura, Seishi Kudo, Yuichi Inoue, Hiroyuki Noji, Kumiko Hayashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The giant acceleration (GA) of diffusion is a universal phenomenon predicted by the theoretical analysis given by Reimann et al. [Phys. Rev. Lett. 87, 010602 (2001)]. Here we apply the theory of the GA of diffusion to a single-molecule experiment on a rotary motor protein, F1, which is a component of FoF1 adenosine triphosphate synthase. We discuss the energetic properties of F1 and identify a high energy barrier of the rotary potential to be 20kBT, with the condition that the adenosine diphosphates are tightly bound to the F1 catalytic sites. To conclude, the GA of diffusion is useful for measuring energy barriers in nonequilibrium and single-molecule experiments.

Original languageEnglish
Article number248101
JournalPhysical Review Letters
Volume114
Issue number24
DOIs
Publication statusPublished - 2015 Jun 16

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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