GFAT as a target molecule of methylmercury toxicity in Saccharomyces cerevisiae

Akira Naganuma, Nobuhiko Miura, Satoshi Kaneko, Tetsuya Mishina, Shinji Hosoya, Shinichi Miyairi, Takemitsu Furuchi, Shusuke Kuge

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Using a genomic library constructed from Saccharomyces cerevisiae, we have identified a gene GFA1 that confers resistance to methylmercury toxicity. GFA1 encodes L-glutamine:D-fructose-6-phosphate amidotransferase (GFAT) and catalyzes synthesis of glucosamine-6-phosphate. Transformed yeast cells expressing GFA1 demonstrated resistance to methylmercury but no resistance to p-chloromercuribenzoate, a GFAT inhibitor. The cytotoxicity of methylmercury was inhibited by loading excess glucosamine 6-phosphate into yeast. Considering that GFAT is an essential cellular enzyme, our findings suggest that GFAT is the major target molecule of methylmercury in yeasts. This report is the first to identify the target molecule of methylmercury toxicity in eukaryotic cells.

Original languageEnglish
Pages (from-to)968-972
Number of pages5
JournalFASEB Journal
Volume14
Issue number7
DOIs
Publication statusPublished - 2000

Keywords

  • Gene screening
  • Glucosamine
  • Glucosamine-6-phosphate
  • P- chloromercuribenzoate
  • Resistance

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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