Geranylgeraniol enhances testosterone production via the cAMP/protein kinase A pathway in testis-derived I-10 tumor cells

Hsin Jung Ho, Hitoshi Shirakawa, Risa Yoshida, Asagi Ito, Misato Maeda, Tomoko Goto, Michio Komai

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Testosterone levels in men decrease with age; this decline has been linked to various diseases and can shorten life expectancy. Geranylgeraniol (GGOH) is an isoprenoid found in plants that plays an important role in several biological processes; however, its role in steroidogenesis is unknown. Here, we report that GGOH enhances the production of testosterone and its precursor progesterone in testis-derived I-10 tumor cells. GGOH induced protein kinase A (PKA) activity and increased cAMP levels and was found to regulate cAMP/PKA signaling by activating adenylate cyclase without altering phosphodiesterase activity. GGOH also stimulated mRNA and protein levels of steroidogenic acute regulatory protein, a downstream effector in the cAMP/PKA pathway. These results demonstrate that GGOH enhances steroidogenesis in testis-derived cells by modulating cAMP/PKA signaling. Our findings have potential applications for the development of therapeutics that increase testosterone levels in aging men.

    Original languageEnglish
    Pages (from-to)791-797
    Number of pages7
    JournalBioscience, Biotechnology and Biochemistry
    Volume80
    Issue number4
    DOIs
    Publication statusPublished - 2016

    Keywords

    • CAMP
    • Geranylgeraniol
    • Progesterone
    • Protein kinase A
    • Testosterone

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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