Geranylgeraniol enhances testosterone production via the cAMP/protein kinase A pathway in testis-derived I-10 tumor cells

Hsin Jung Ho, Hitoshi Shirakawa, Risa Yoshida, Asagi Ito, Misato Maeda, Tomoko Goto, Michio Komai

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Testosterone levels in men decrease with age; this decline has been linked to various diseases and can shorten life expectancy. Geranylgeraniol (GGOH) is an isoprenoid found in plants that plays an important role in several biological processes; however, its role in steroidogenesis is unknown. Here, we report that GGOH enhances the production of testosterone and its precursor progesterone in testis-derived I-10 tumor cells. GGOH induced protein kinase A (PKA) activity and increased cAMP levels and was found to regulate cAMP/PKA signaling by activating adenylate cyclase without altering phosphodiesterase activity. GGOH also stimulated mRNA and protein levels of steroidogenic acute regulatory protein, a downstream effector in the cAMP/PKA pathway. These results demonstrate that GGOH enhances steroidogenesis in testis-derived cells by modulating cAMP/PKA signaling. Our findings have potential applications for the development of therapeutics that increase testosterone levels in aging men.

Original languageEnglish
Pages (from-to)791-797
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number4
DOIs
Publication statusPublished - 2016

Keywords

  • CAMP
  • Geranylgeraniol
  • Progesterone
  • Protein kinase A
  • Testosterone

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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