Geranyl modification on the tryptophan residue of ComX RO-E-2 pheromone by a cell-free system

Fumitada Tsuji, Ayako Ishihara, Keiko Kurata, Aya Nakagawa, Masahiro Okada, Shigeyuki Kitamura, Kyoko Kanamaru, Yuichi Masuda, Kazuma Murakami, Kazuhiro Irie, Youji Sakagami

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalFEBS Letters
Volume586
Issue number2
DOIs
Publication statusPublished - 2012 Jan 20

Keywords

  • Bacillus subtilis
  • ComX pheromone
  • Posttranslational modification
  • Prenylation
  • Prenyltransferase
  • Quorum sensing

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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