Everted intestinal sacs were made from rainbow trout, Salmo gairdneri, and were used to in-vestigate the intestinal transport of L-lysine-14C, in vitro. With incubation for 2h at 37±1°C, L-Lysine was transported across the mid and posterior intestine. The uptake ratio of the mid intestine varied from 6.68 % to 26.64 % during the incubation period. Similarly, the ratio of the posterior segment also increased from 1.62% to 23.24%. The presence of 10 mM glucose reduced the uptake of L-Lysine from 26.64% to 12.34% in the mid intestine and from 23.24% to 5.40% in the posterior one. 1 mM ATP increased the uptake ratio of L-Iysine from 26.64% to 41.72% in the mid intestine and from 23.24% to 32.92% in the posterior one. L-lysine transport by the fish intestine was stimulated by 1 mM ATP but inhibited by 10 mM glucose. The intestine of the rain-bow trout exhibited nearly the same transport activity for L-lysine between the two intestinal seg-ments for 20 mM glucose and 1 mM ATP.
ASJC Scopus subject areas
- Aquatic Science