Genotyping of single nucleotide polymorphisms (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.

Masahiro Hiratsuka, Aiko Ebisawa, Yoichi Matsubara, Shigeo Kure, Yumiko Konno, Takamitsu Sasaki, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Using competitive allele-specific oligonucleotide hybridization with immunochromatographic strip (CASSOH), we have developed a simplified method for the detection of eight polymorphisms that are especially important in the identification of drug responders or non-responders and patients at increased risk of drug toxicity. The genotyping method is unambiguously determined by the presence or the absence of visible purple lines on the immunochromatographic strip, and results are obtained within 5 min after PCR. This method is rapid, highly sensitive, simplified, and should be suitable for point-of-care genotyping in clinical settings.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalDrug metabolism and pharmacokinetics
Volume19
Issue number4
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

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