TY - JOUR
T1 - Genotyping of respiratory syncytial virus among influenza-like illness and severe acute respiratory infection cases of children in the Philippines from 2006 to 2016
AU - Calaor-Morin, Jonjee
AU - Arguelles, Vina Lea
AU - Foronda, Janiza Lianne
AU - Tan, Alvin
AU - Lagamayo, Evelina
AU - Dapat, Clyde
AU - Lupisan, Socorro
N1 - Funding Information:
The authors thanks Dr Irma Asuncion (DOH‐EB), and Dr Lyndon Lee‐Suy and Dr Gemma Arellano (Disease Prevention and Control Bureau) for providing support to this study; Ava Kristy S. Lee for her contribution during the development and implementation of the study; RITM Virology Department and Data Management Unit (DMU) for the technical assistance and data storage; and Mary Lorraine Mationg and Johanna Beulah Sornillo for the technical assistance in sampling and data analysis. This study was financially supported by the DOH Disease Prevention and Control Bureau (DPCB) and Epidemiology Bureau (EB) in the Philippines (SAA‐2017‐04‐209).
Funding Information:
The authors thanks Dr Irma Asuncion (DOH-EB), and Dr Lyndon Lee-Suy and Dr Gemma Arellano (Disease Prevention and Control Bureau) for providing support to this study; Ava Kristy S. Lee for her contribution during the development and implementation of the study; RITM Virology Department and Data Management Unit (DMU) for the technical assistance and data storage; and Mary Lorraine Mationg and Johanna Beulah Sornillo for the technical assistance in sampling and data analysis. This study was financially supported by the DOH Disease Prevention and Control Bureau (DPCB) and Epidemiology Bureau (EB) in the Philippines (SAA-2017-04-209).
Publisher Copyright:
© 2022 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
PY - 2022/9
Y1 - 2022/9
N2 - Objective: Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory infection, and therefore, a major threat to global health. This study determined the epidemiological and molecular characteristics of RSV among cases of influenza-like illness (ILI) and severe acute respiratory infection (SARI) among children in the Philippines. Method: The study included archived nasopharyngeal swab and oropharyngeal swab samples collected from patients under the age of five who are presented with ILI or SARI for the period of 2006–2016. Swabs were examined for RSV subgroup by multiplex real-time qRT-PCR. Partial genome sequencing and phylogenetic analyses of the second hypervariable region (HVR) of the G gene were used to determine the genotype of RSV isolates. Results: A total of 1036 representative samples from all sites were selected and tested. Of these samples, 122 were RSV-positive at 11.8% prevalence rate, and 58.2% (71/122) were classified as RSV-A. Six genotypes were identified, which include NA1 (27/122, 22.1%), ON1 (5/122, 4.1%), GA2 (1/122, 0.8%), and GA5 (1/122, 0.8%) for RSV-A; and BA2 (13/122, 10.7%) and BA9 (1/122, 0.8%) for RSV-B. Most RSV-related cases were significantly associated with clinical characteristics such as runny nose (88.1% RSV vs. 11.9% non-RSV: p value = 0.021), pneumonia (80.6% RSV vs. 19.4% non-RSV; p value = 0.015), and bronchitis (71.7% RSV vs. 28.3% non-RSV; p value < 0.001). Increased RSV-related cases were observed among children below 24 months old. Conclusion: The RSV trend and genetic variability in the Philippines resembles a similar pattern of transmission globally.
AB - Objective: Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory infection, and therefore, a major threat to global health. This study determined the epidemiological and molecular characteristics of RSV among cases of influenza-like illness (ILI) and severe acute respiratory infection (SARI) among children in the Philippines. Method: The study included archived nasopharyngeal swab and oropharyngeal swab samples collected from patients under the age of five who are presented with ILI or SARI for the period of 2006–2016. Swabs were examined for RSV subgroup by multiplex real-time qRT-PCR. Partial genome sequencing and phylogenetic analyses of the second hypervariable region (HVR) of the G gene were used to determine the genotype of RSV isolates. Results: A total of 1036 representative samples from all sites were selected and tested. Of these samples, 122 were RSV-positive at 11.8% prevalence rate, and 58.2% (71/122) were classified as RSV-A. Six genotypes were identified, which include NA1 (27/122, 22.1%), ON1 (5/122, 4.1%), GA2 (1/122, 0.8%), and GA5 (1/122, 0.8%) for RSV-A; and BA2 (13/122, 10.7%) and BA9 (1/122, 0.8%) for RSV-B. Most RSV-related cases were significantly associated with clinical characteristics such as runny nose (88.1% RSV vs. 11.9% non-RSV: p value = 0.021), pneumonia (80.6% RSV vs. 19.4% non-RSV; p value = 0.015), and bronchitis (71.7% RSV vs. 28.3% non-RSV; p value < 0.001). Increased RSV-related cases were observed among children below 24 months old. Conclusion: The RSV trend and genetic variability in the Philippines resembles a similar pattern of transmission globally.
KW - RSV-A
KW - RSV-B
KW - genotype shifts
KW - genotype variations
KW - influenza-like illness
KW - severe acute respiratory infection
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UR - http://www.scopus.com/inward/citedby.url?scp=85130306458&partnerID=8YFLogxK
U2 - 10.1111/irv.12986
DO - 10.1111/irv.12986
M3 - Article
C2 - 35582932
AN - SCOPUS:85130306458
SN - 1750-2640
VL - 16
SP - 942
EP - 951
JO - Influenza and other Respiratory Viruses
JF - Influenza and other Respiratory Viruses
IS - 5
ER -
