Genotype analysis of prepro-vasopressin signal peptide in vasopressin- producing and -non-producing lung tumors

Masaru Shoji, Tokihisa Kimura, Kozo Ota, Tohru Yamaji, Miyuki Ishibashi, Masahiro Ohta, Hironobu Sasano, Yutaka Kawarabayasi, Noriko Kimura, Takeharu Funyu, Takefumi Mori, Takayuki Sahata

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A polymorphism in the nucleic acid sequence encoding the signal peptide of the human prepro-vasopressin (AVP) has been reported in an AVP producing small cell lung carcinoma (SCLC) cell line. The difference predicts expression in tumor cells of a variant signal peptide with Pro for Leu 11. To clarify whether this difference is required for AVP secretion from SCLC cells and/or reflects increased mutagenesis in malignant tumors, the exon encoding the signal peptide of prepro-AVP in two AVP producing SCLC and 9 non- producing lung tumors was amplified using polymerase chain reaction. The variant sequence was neither found by direct sequencing nor by restriction enzyme analysis. These results suggest that similar to the hypothalamus the normal signal peptide is functional in tumor cells and that the variant signal peptide is not a prerequisite for AVP secretion from SCLC cells.

Original languageEnglish
Pages (from-to)2561-2566
Number of pages6
JournalLife Sciences
Volume61
Issue number26
DOIs
Publication statusPublished - 1997 Nov 21

Keywords

  • Direct sequencing
  • Polymerase chain reaction
  • Restriction enzyme
  • Small cell lung cancer
  • Vasopressin signal peptide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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