Genomic organization of the AODEF gene in Asparagus officinalis L.

Takuro Ito, Go Suzuki, Toshinori Ochiai, Mutsumi Nakada, Toshiaki Kameya, Akira Kanno

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The perianths of Liliaceae plants, such as lily and tulip, have two whorls of almost identical petaloid organs, which are called tepals. According to the modified ABC model proposed in tulip, the class B genes are expressed in whorl 1 as well as whorls 2 and 3, so that the organs of whorls 1 and 2 have the same petaloid structure. The floral structure of asparagus (Asparagus officinalis L.) is similar to that of Liliaceae plants, however, the expression of B-class genes (AODEF, AOGLOA, AOGLOB) was not found in whorl 1, but was confined to whorls 2 and 3. This result does not support the modified ABC model in asparagus. In order to gain a better understanding of asparagus flower development, we have characterized a genomic clone of the AODEF gene. We compared the genomic organization and promoter sequence of AODEF with three well-studied DEF-like genes, DEFICIENS (Antirrhinum), APETALA3 (Arabidopsis), and OSMADS16 (rice). Exon-intron structures of these genes are well-conserved except for the large fifth intron in the AODEF gene and the OSMADS16 gene. Putative cis-elements including CArG-boxes were found in the promoter region and forty-two microsatellites were found in the AODEF genomic sequence.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalGenes and Genetic Systems
Volume80
Issue number2
DOIs
Publication statusPublished - 2005

Keywords

  • AODEF
  • Asparagus officinalis
  • Cis-element
  • Genomic sequence
  • Microsatellite

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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