Genomic organization, chromosomal localization, and the complete 22 kb DNA sequence of the human GCMa/GCM1, a placenta-specific transcription factor gene

Kazuyo Yamada, Hisamitsu Ogawa, Gen Tamiya, Masashi Ikeno, Miwa Morita, Shuichi Asakawa, Nobuyoshi Shimizu, Tuneko Okazaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The genomic sequence of the human GCMa/GCM1 gene, a mammalian homologue of Drosophila melanogaster GCM, was determined. Drosophila GCM is a neural transcription factor that regulates glial cell fate. The mammalian homolog however, is a placenta-specific transcription factor that is necessary for placental development. The 22 kb DNA sequence spanning the GCMa gene contains six exons and five introns, encoding a 2.8 kb cDNA. Overall genomic organization is similar for the human and mouse. Several potential binding sites for transcription factors like GATA, Oct-1, and bHLH proteins were found in the 5'-flanking region of the human gene. A DNA motif for GCM protein binding exists in the 5'-flanking region that is highly homologous with that of the mouse gene. The location of this gene was mapped to chromosome 6 using fluorescence in situ hybridization. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalBiochemical and biophysical research communications
Volume278
Issue number1
DOIs
Publication statusPublished - 2000 Nov 11

Keywords

  • GCMa/GCM1
  • Genomic organization
  • Placenta
  • Transcription factor
  • Trophoblast Specific Element

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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