Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene

Hiroyuki Minekura, Man Jong Kang, Yosuke Inagaki, Hiroyuki Suzuki, Hiroyuki Sato, Takahiro Fujino, Tokuo T. Yamamoto

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Acyl-CoA synthetases (ACSs) play an essential role in fatty acid metabolism. ACS3 is an arachidonate-preferring enzyme expressed in a wide range of human tissues including brain, heart, placenta, prostate, skeletal muscle, testis and thymus. As an initial step to understanding the transcriptional regulation of the human ACS3 gene, we analyzed the genomic organization and transcription units of the human ACS3 gene. Sequence analysis of genomic clones demonstrates that the human ACS3 gene spans at least 80.6 kb and contains 17 exons. The human ACS3 gene was mapped between the sequence-tagged site markers D2S360 and WI-21901. Sequence inspection of the 5′-flanking region revealed potential DNA elements including CCAAT, AP-1, Oct-1, GATAs, SRY, CdxA, Nkx-2.5, c-Myb, HSF2, NF-AT, AP-2, NF-Y, and p300. A minimal promoter region required for the expression of the human ACS3 gene in melanoma G361 cells was determined.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalGene
Volume278
Issue number1-2
DOIs
Publication statusPublished - 2001 Oct 31

Keywords

  • Acyl-CoA synthetase
  • Arachidonate
  • Fatty acid
  • Genomic organization

ASJC Scopus subject areas

  • Genetics

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