Genomic organisation and characterisation of the neural sex-determination gene fruitless (fru) in the Hawaiian species Drosophila heteroneura

Terence Davis, Joyce Kurihara, Daisuke Yamamoto

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    There are several mechanisms for the determination of sex. Sexual behaviour is part of the sex-determination cascade, and in Drosophila melanogaster male courtship is controlled in part by the fruitless gene. As part of a study of sexual behaviour in Hawaiian Drosophila, we have cloned the neural sex-determination gene fru from the Hawaiian picture-wing species Drosophila heteroneura. The fru gene has at least seven exons covering a region of 18 kb and encodes three transcripts, fruA, fruB and fruC. Each transcript encodes a single ORF of 841, 678 and 691 aa, respectively. The FRUA and FRUB proteins have a BTB protein- protein-binding domain and two zinc finger-like domains and are well conserved with the D. melanogaster proteins. The FRUC protein has a BTB domain but no zinc finger-like domains. The fru gene is expressed in 1-7 day old adult males as a 5.1 kb transcript. This transcript is not seen in adult females, so the fru gene has a different pattern of sex-differential expression in the Hawaiian Drosophila compared with D. melanogaster. (C) 2000 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)143-149
    Number of pages7
    JournalGene
    Volume246
    Issue number1-2
    DOIs
    Publication statusPublished - 2000 Apr 4

    Keywords

    • BTB protein-protein-binding domain
    • DNA cloning
    • Muscle of Lawrence
    • Sexual behaviour
    • Transcription factor
    • Zinc finger domain
    • bp, base pairs
    • kb, 1000-base pairs

    ASJC Scopus subject areas

    • Genetics

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