Genomic and serological detection of bat coronavirus from bats in the Philippines

Shumpei Tsuda; Shumpei Watanabe; Joseph S. Masangkay; Tetsuya Mizutani; Phillip Alviola; Naoya Ueda; Koichiro Iha; Satoshi Taniguchi; Hikaru Fujii; Kentaro Kato; Taisuke Horimoto; Shigeru Kyuwa; Yasuhiro Yoshikawa; Hiroomi Akashi

doi:10.1007/s00705-012-1410-z

Genomic and serological detection of bat coronavirus from bats in the Philippines

Shumpei Tsuda, Shumpei Watanabe, Joseph S. Masangkay, Tetsuya Mizutani, Phillip Alviola, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Kentaro Kato, Taisuke Horimoto, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.

Original language	English
Pages (from-to)	2349-2355
Number of pages	7
Journal	Archives of virology
Volume	157
Issue number	12
DOIs	https://doi.org/10.1007/s00705-012-1410-z
Publication status	Published - 2012 Dec
Externally published	Yes

Keywords

Antibody
Bat coronavirus
Bats
Coronavirus
SARS
Severe acute respiratory syndrome
Viruses
Zoonosis

ASJC Scopus subject areas

Virology

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10.1007/s00705-012-1410-z

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title = "Genomic and serological detection of bat coronavirus from bats in the Philippines",

abstract = "Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.",

keywords = "Antibody, Bat coronavirus, Bats, Coronavirus, SARS, Severe acute respiratory syndrome, Viruses, Zoonosis",

author = "Shumpei Tsuda and Shumpei Watanabe and Masangkay, {Joseph S.} and Tetsuya Mizutani and Phillip Alviola and Naoya Ueda and Koichiro Iha and Satoshi Taniguchi and Hikaru Fujii and Kentaro Kato and Taisuke Horimoto and Shigeru Kyuwa and Yasuhiro Yoshikawa and Hiroomi Akashi",

note = "Funding Information: We greatly thank Mr. Edison Cosico and Mr. Eduardo Eres for the arrangements for bat collection. This study was supported by a grant from a Ministry of Education, Culture, Sports, Science and Technology, Japan.",

year = "2012",

month = dec,

doi = "10.1007/s00705-012-1410-z",

language = "English",

volume = "157",

pages = "2349--2355",

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TY - JOUR

T1 - Genomic and serological detection of bat coronavirus from bats in the Philippines

AU - Tsuda, Shumpei

AU - Watanabe, Shumpei

AU - Masangkay, Joseph S.

AU - Mizutani, Tetsuya

AU - Alviola, Phillip

AU - Ueda, Naoya

AU - Iha, Koichiro

AU - Taniguchi, Satoshi

AU - Fujii, Hikaru

AU - Kato, Kentaro

AU - Horimoto, Taisuke

AU - Kyuwa, Shigeru

AU - Yoshikawa, Yasuhiro

AU - Akashi, Hiroomi

N1 - Funding Information: We greatly thank Mr. Edison Cosico and Mr. Eduardo Eres for the arrangements for bat collection. This study was supported by a grant from a Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2012/12

Y1 - 2012/12

N2 - Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.

AB - Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.

KW - Antibody

KW - Bat coronavirus

KW - Bats

KW - Coronavirus

KW - SARS

KW - Severe acute respiratory syndrome

KW - Viruses

KW - Zoonosis

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ER -

Genomic and serological detection of bat coronavirus from bats in the Philippines

Abstract

Keywords

ASJC Scopus subject areas

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