Genomic and serological detection of bat coronavirus from bats in the Philippines

Shumpei Tsuda, Shumpei Watanabe, Joseph S. Masangkay, Tetsuya Mizutani, Phillip Alviola, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Kentaro Kato, Taisuke Horimoto, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.

Original languageEnglish
Pages (from-to)2349-2355
Number of pages7
JournalArchives of virology
Volume157
Issue number12
DOIs
Publication statusPublished - 2012 Dec
Externally publishedYes

Keywords

  • Antibody
  • Bat coronavirus
  • Bats
  • Coronavirus
  • SARS
  • Severe acute respiratory syndrome
  • Viruses
  • Zoonosis

ASJC Scopus subject areas

  • Virology

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