TY - JOUR
T1 - Genomic analysis of the thymine-DNA glycosylase (TDG) gene on 12q22- q24.1 in human pancreatic ductal adenocarcinoma
AU - Yatsuoka, Toshimasa
AU - Furukawa, Toru
AU - Abe, Tadayoshi
AU - Yokoyama, Tadaaki
AU - Sunamura, Makoto
AU - Kobari, Masao
AU - Matsuno, Seiki
AU - Horii, Akira
PY - 1999
Y1 - 1999
N2 - Background. Recently, a human G/T-specific TDG gene was identified. This protein acts in a system correcting G/T mispairs to G/C pairs. TDG was mapped to chromosome bands 12q22-q24.1, one of the regions frequently lost in pancreatic cancer. Therefore, there is the possibility that the TDG gene on 12q is one of the genes responsible for pancreatic ductal carcinogenesis. Methods. Nucleotide sequences of the entire coding region of the TDG gene were analyzed in 21 human pancreatic cancer cell lines, mRNA expression of the TDG gene was also analyzed by Northern hybridization in several human tissues and 21 human pancreatic cancer celt lines. Results. Decreased levels of mRNA expression were detected in the pancreatic cancer cell lines, but no somatic mutations were observed. Conclusion. Abnormality of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 appears to play a limited role in pancreatic ductal carcinogenesis.
AB - Background. Recently, a human G/T-specific TDG gene was identified. This protein acts in a system correcting G/T mispairs to G/C pairs. TDG was mapped to chromosome bands 12q22-q24.1, one of the regions frequently lost in pancreatic cancer. Therefore, there is the possibility that the TDG gene on 12q is one of the genes responsible for pancreatic ductal carcinogenesis. Methods. Nucleotide sequences of the entire coding region of the TDG gene were analyzed in 21 human pancreatic cancer cell lines, mRNA expression of the TDG gene was also analyzed by Northern hybridization in several human tissues and 21 human pancreatic cancer celt lines. Results. Decreased levels of mRNA expression were detected in the pancreatic cancer cell lines, but no somatic mutations were observed. Conclusion. Abnormality of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 appears to play a limited role in pancreatic ductal carcinogenesis.
KW - Chromosome 12q
KW - DNA mismatch repair system
KW - LOH
KW - Pancreatic ductal carcinogenesis
KW - The G/T-specific thymine-DNA glycosylase (TDG) gene
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U2 - 10.1385/ijgc:25:2:97
DO - 10.1385/ijgc:25:2:97
M3 - Article
C2 - 10360221
AN - SCOPUS:0033036074
VL - 25
SP - 97
EP - 102
JO - Journal of Gastrointestinal Cancer
JF - Journal of Gastrointestinal Cancer
SN - 1941-6628
IS - 2
ER -