Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer

Hitoshi Hiura, Hiromitsu Hattori, Norio Kobayashi, Hiroaki Okae, Hatsune Chiba, Naoko Miyauchi, Akane Kitamura, Hiroyuki Kikuchi, Hiroaki Yoshida, Takahiro Arima

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Background: Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension (PIH). Results: In this study, we compared the microRNA (miRNA) expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy (SP). We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus (T2DM) were enriched. Conclusions: This study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences.

Original languageEnglish
Article number79
JournalClinical Epigenetics
Issue number1
Publication statusPublished - 2017 Aug 3


  • Assisted reproductive technologies (ART)
  • Frozen-thawed embryo transfer (FET)
  • MicroRNAs (miRNAs)
  • Microarray
  • Placenta
  • Real-time PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology
  • Genetics(clinical)


Dive into the research topics of 'Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer'. Together they form a unique fingerprint.

Cite this