TY - JOUR
T1 - Genome-wide identification, structure and expression studies, and mutant collection of 22 early nodulin-like protein genes in arabidopsis
AU - Mashiguchi, Kiyoshi
AU - Asami, Tadao
AU - Suzuki, Yoshihito
N1 - Funding Information:
We are grateful to the ABRC, GABI-Kat, and NASC for providing plant material, and to Atsutaka Kato for assistance in preparing plant materials. This work was supported in part by grants from the program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) (to T.A.), and Grants-in-Aid for Scientific Research by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (no. 17380071, to Y.S), and the Japan Society of the Promotion of Science Fellows (no. 12046, to K.M).
PY - 2009/11
Y1 - 2009/11
N2 - Early nodulin-like proteins (ENODLs) are chimeric arabinogalactan proteins (AGPs) related to the phytocyanin family. Although they show similarities with other phytocyanins, they lack amino acid residues for copper binding. Despite the existence of other phytocyanins, information about the function of ENODLs in plants is largely lacking. In this study, we characterized ENODL genes consisting of 22 members in Arabidopsis thaliana. Structure prediction indicated that most ENODLs are glycosylphosphatidylinositol-anchored chimeric AGPs. Expression analysis by real-time reverse transcription polymerase chain reaction indicated that most ENODL genes showed spatially specific expression, mainly in the flower organs. Furthermore, we obtained and analyzed 26 homozygous T-DNA insertion lines of 15 ENODL genes, but novel biological roles were not uncovered, probably due to functional redundancy. The detailed phylogenetic and expression analyses and characterization of the available insertion lines in this study might facilitate future studies to elucidate the biological roles of ENODLs.
AB - Early nodulin-like proteins (ENODLs) are chimeric arabinogalactan proteins (AGPs) related to the phytocyanin family. Although they show similarities with other phytocyanins, they lack amino acid residues for copper binding. Despite the existence of other phytocyanins, information about the function of ENODLs in plants is largely lacking. In this study, we characterized ENODL genes consisting of 22 members in Arabidopsis thaliana. Structure prediction indicated that most ENODLs are glycosylphosphatidylinositol-anchored chimeric AGPs. Expression analysis by real-time reverse transcription polymerase chain reaction indicated that most ENODL genes showed spatially specific expression, mainly in the flower organs. Furthermore, we obtained and analyzed 26 homozygous T-DNA insertion lines of 15 ENODL genes, but novel biological roles were not uncovered, probably due to functional redundancy. The detailed phylogenetic and expression analyses and characterization of the available insertion lines in this study might facilitate future studies to elucidate the biological roles of ENODLs.
KW - Arabidopsis
KW - Arabinogalactan protein
KW - Early nodulin-like protein
KW - Glycosylphosphatidylinositol-anchored protein
KW - Phytocyanin
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U2 - 10.1271/bbb.90407
DO - 10.1271/bbb.90407
M3 - Article
C2 - 19897921
AN - SCOPUS:72949118524
VL - 73
SP - 2452
EP - 2459
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -