Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus

Naoki Iwanaga, Kaori Ide, Takeshi Nagashima, Takeo Tomita, Yoshihiro Agari, Akeo Shinkai, Seiki Kuramitsu, Mariko Okada-Hatakeyema, Tomohisa Kuzuyama, Makoto Nishiyama

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6 Citations (Scopus)

Abstract

ArgR is known to serve as a repressor/activator of the metabolism of arginine. To elucidate the role of ArgR in the metabolism of Thermus thermophilus cells, comparative genome-wide comprehensive analysis was conducted for wild-type T. thermophilus and its mutant lacking the argR gene. Transcriptome analysis and chromatin affinity precipitation coupled with high-density tiling chip (ChAP-chip) analysis identified 34 genetic loci that are directly regulated by ArgR and indicated that ArgR decreases the expression of arginine biosynthesis and also regulates several other genes involved in amino acid metabolism, including lysine biosynthetic genes, as suggested by our previous study. Among genes whose expression was regulated by ArgR, the largest effect of argR knockout was observed in a putative operon, including genes TTHA0284, TTHA0283, and TTHA0282 involved in arginine biosynthesis. The promoter of this operon, argG, was repressed approximately 21-fold by ArgR. DNase I footprint analysis coupled with electrophoretic mobility shift assay suggested that high arginine-dependent repression was attributed to the fact that the promoter contains three operators for ArgR binding and ArgR is bound to the binding sites cooperatively, possibly forming a DNA loop, in the hexameric form stabilized by arginine binding.

Original languageEnglish
JournalExtremophiles
DOIs
Publication statusAccepted/In press - 2014 Jul 29

Keywords

  • argR
  • DNA microarray
  • Thermus thermophilus
  • Transcriptional regulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Medicine

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  • Cite this

    Iwanaga, N., Ide, K., Nagashima, T., Tomita, T., Agari, Y., Shinkai, A., Kuramitsu, S., Okada-Hatakeyema, M., Kuzuyama, T., & Nishiyama, M. (Accepted/In press). Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus. Extremophiles. https://doi.org/10.1007/s00792-014-0669-2