Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki; Tatsuma Okazaki; Kurisu Tada; Sachiko Sakon-Komazawa; Mitsuo Katano; Masao Tanaka; Hideo Yagita; Ko Okumura; Naoko Tominaga; Yoshihide Hayashizaki; Yasushi Okazaki; Hiroyasu Nakano

doi:10.1016/j.molimm.2004.03.030

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki, Tatsuma Okazaki, Kurisu Tada, Sachiko Sakon-Komazawa, Mitsuo Katano, Masao Tanaka, Hideo Yagita, Ko Okumura, Naoko Tominaga, Yoshihide Hayashizaki, Yasushi Okazaki, Hiroyasu Nakano

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Abstract

We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

Original language	English
Pages (from-to)	547-551
Number of pages	5
Journal	Molecular Immunology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1016/j.molimm.2004.03.030
Publication status	Published - 2004 Jul

Keywords

Microarray
NF-κB
ROS
TNF
TRAF

ASJC Scopus subject areas

Immunology
Molecular Biology

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Sasazuki, T., Okazaki, T., Tada, K., Sakon-Komazawa, S., Katano, M., Tanaka, M., Yagita, H., Okumura, K., Tominaga, N., Hayashizaki, Y., Okazaki, Y., & Nakano, H. (2004). Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. Molecular Immunology, 41(5), 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. / Sasazuki, Tomonari; Okazaki, Tatsuma; Tada, Kurisu; Sakon-Komazawa, Sachiko; Katano, Mitsuo; Tanaka, Masao; Yagita, Hideo; Okumura, Ko; Tominaga, Naoko; Hayashizaki, Yoshihide; Okazaki, Yasushi; Nakano, Hiroyasu.

In: Molecular Immunology, Vol. 41, No. 5, 07.2004, p. 547-551.

Research output: Contribution to journal › Article › peer-review

Sasazuki, T, Okazaki, T, Tada, K, Sakon-Komazawa, S, Katano, M, Tanaka, M, Yagita, H, Okumura, K, Tominaga, N, Hayashizaki, Y, Okazaki, Y & Nakano, H 2004, 'Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS', Molecular Immunology, vol. 41, no. 5, pp. 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

Sasazuki, Tomonari ; Okazaki, Tatsuma ; Tada, Kurisu ; Sakon-Komazawa, Sachiko ; Katano, Mitsuo ; Tanaka, Masao ; Yagita, Hideo ; Okumura, Ko ; Tominaga, Naoko ; Hayashizaki, Yoshihide ; Okazaki, Yasushi ; Nakano, Hiroyasu. / Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. In: Molecular Immunology. 2004 ; Vol. 41, No. 5. pp. 547-551.

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abstract = "We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.",

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note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).",

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AU - Okumura, Ko

AU - Tominaga, Naoko

AU - Hayashizaki, Yoshihide

AU - Okazaki, Yasushi

AU - Nakano, Hiroyasu

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).

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AB - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

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