Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki; Tatsuma Okazaki; Kurisu Tada; Sachiko Sakon-Komazawa; Mitsuo Katano; Masao Tanaka; Hideo Yagita; Ko Okumura; Naoko Tominaga; Yoshihide Hayashizaki; Yasushi Okazaki; Hiroyasu Nakano

doi:10.1016/j.molimm.2004.03.030

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki, Tatsuma Okazaki, Kurisu Tada, Sachiko Sakon-Komazawa, Mitsuo Katano, Masao Tanaka, Hideo Yagita, Ko Okumura, Naoko Tominaga, Yoshihide Hayashizaki, Yasushi Okazaki, Hiroyasu Nakano

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

Original language	English
Pages (from-to)	547-551
Number of pages	5
Journal	Molecular Immunology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1016/j.molimm.2004.03.030
Publication status	Published - 2004 Jul
Externally published	Yes

Keywords

Microarray
NF-κB
ROS
TNF
TRAF

ASJC Scopus subject areas

Immunology
Molecular Biology

Access to Document

10.1016/j.molimm.2004.03.030

Cite this

Sasazuki, T., Okazaki, T., Tada, K., Sakon-Komazawa, S., Katano, M., Tanaka, M., Yagita, H., Okumura, K., Tominaga, N., Hayashizaki, Y., Okazaki, Y., & Nakano, H. (2004). Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. Molecular Immunology, 41(5), 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. / Sasazuki, Tomonari; Okazaki, Tatsuma; Tada, Kurisu; Sakon-Komazawa, Sachiko; Katano, Mitsuo; Tanaka, Masao; Yagita, Hideo; Okumura, Ko; Tominaga, Naoko; Hayashizaki, Yoshihide; Okazaki, Yasushi; Nakano, Hiroyasu.

In: Molecular Immunology, Vol. 41, No. 5, 07.2004, p. 547-551.

Research output: Contribution to journal › Article › peer-review

Sasazuki, T, Okazaki, T, Tada, K, Sakon-Komazawa, S, Katano, M, Tanaka, M, Yagita, H, Okumura, K, Tominaga, N, Hayashizaki, Y, Okazaki, Y & Nakano, H 2004, 'Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS', Molecular Immunology, vol. 41, no. 5, pp. 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

Sasazuki, Tomonari ; Okazaki, Tatsuma ; Tada, Kurisu ; Sakon-Komazawa, Sachiko ; Katano, Mitsuo ; Tanaka, Masao ; Yagita, Hideo ; Okumura, Ko ; Tominaga, Naoko ; Hayashizaki, Yoshihide ; Okazaki, Yasushi ; Nakano, Hiroyasu. / Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. In: Molecular Immunology. 2004 ; Vol. 41, No. 5. pp. 547-551.

@article{c8f21449b73c4292ac8afd89166c5a23,

title = "Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS",

abstract = "We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.",

keywords = "Microarray, NF-κB, ROS, TNF, TRAF",

author = "Tomonari Sasazuki and Tatsuma Okazaki and Kurisu Tada and Sachiko Sakon-Komazawa and Mitsuo Katano and Masao Tanaka and Hideo Yagita and Ko Okumura and Naoko Tominaga and Yoshihide Hayashizaki and Yasushi Okazaki and Hiroyasu Nakano",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).",

year = "2004",

month = jul,

doi = "10.1016/j.molimm.2004.03.030",

language = "English",

volume = "41",

pages = "547--551",

journal = "Immunochemistry",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "5",

}

TY - JOUR

T1 - Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

AU - Sasazuki, Tomonari

AU - Okazaki, Tatsuma

AU - Tada, Kurisu

AU - Sakon-Komazawa, Sachiko

AU - Katano, Mitsuo

AU - Tanaka, Masao

AU - Yagita, Hideo

AU - Okumura, Ko

AU - Tominaga, Naoko

AU - Hayashizaki, Yoshihide

AU - Okazaki, Yasushi

AU - Nakano, Hiroyasu

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).

PY - 2004/7

Y1 - 2004/7

N2 - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

AB - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

KW - Microarray

KW - NF-κB

KW - ROS

KW - TNF

KW - TRAF

UR - http://www.scopus.com/inward/record.url?scp=2942593988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942593988&partnerID=8YFLogxK

U2 - 10.1016/j.molimm.2004.03.030

DO - 10.1016/j.molimm.2004.03.030

M3 - Article

C2 - 15183933

AN - SCOPUS:2942593988

VL - 41

SP - 547

EP - 551

JO - Immunochemistry

JF - Immunochemistry

SN - 0161-5890

IS - 5

ER -

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this