Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki; Tatsuma Okazaki; Kurisu Tada; Sachiko Sakon-Komazawa; Mitsuo Katano; Masao Tanaka; Hideo Yagita; Ko Okumura; Naoko Tominaga; Yoshihide Hayashizaki; Yasushi Okazaki; Hiroyasu Nakano

doi:10.1016/j.molimm.2004.03.030

Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki, Tatsuma Okazaki, Kurisu Tada, Sachiko Sakon-Komazawa, Mitsuo Katano, Masao Tanaka, Hideo Yagita, Ko Okumura, Naoko Tominaga, Yoshihide Hayashizaki, Yasushi Okazaki, Hiroyasu Nakano

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

Original language	English
Pages (from-to)	547-551
Number of pages	5
Journal	Molecular Immunology
Volume	41
Issue number	5
DOIs	https://doi.org/10.1016/j.molimm.2004.03.030
Publication status	Published - 2004 Jul
Externally published	Yes

Keywords

Microarray
NF-κB
ROS
TNF
TRAF

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/j.molimm.2004.03.030

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Sasazuki, T., Okazaki, T., Tada, K., Sakon-Komazawa, S., Katano, M., Tanaka, M., Yagita, H., Okumura, K., Tominaga, N., Hayashizaki, Y., Okazaki, Y., & Nakano, H. (2004). Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS. Molecular Immunology, 41(5), 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

Sasazuki, T, Okazaki, T, Tada, K, Sakon-Komazawa, S, Katano, M, Tanaka, M, Yagita, H, Okumura, K, Tominaga, N, Hayashizaki, Y, Okazaki, Y & Nakano, H 2004, 'Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS', Molecular Immunology, vol. 41, no. 5, pp. 547-551. https://doi.org/10.1016/j.molimm.2004.03.030

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title = "Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS",

abstract = "We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.",

keywords = "Microarray, NF-κB, ROS, TNF, TRAF",

author = "Tomonari Sasazuki and Tatsuma Okazaki and Kurisu Tada and Sachiko Sakon-Komazawa and Mitsuo Katano and Masao Tanaka and Hideo Yagita and Ko Okumura and Naoko Tominaga and Yoshihide Hayashizaki and Yasushi Okazaki and Hiroyasu Nakano",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).",

year = "2004",

month = jul,

doi = "10.1016/j.molimm.2004.03.030",

language = "English",

volume = "41",

pages = "547--551",

journal = "Immunochemistry",

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T1 - Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

AU - Sasazuki, Tomonari

AU - Okazaki, Tatsuma

AU - Tada, Kurisu

AU - Sakon-Komazawa, Sachiko

AU - Katano, Mitsuo

AU - Tanaka, Masao

AU - Yagita, Hideo

AU - Okumura, Ko

AU - Tominaga, Naoko

AU - Hayashizaki, Yoshihide

AU - Okazaki, Yasushi

AU - Nakano, Hiroyasu

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).

PY - 2004/7

Y1 - 2004/7

N2 - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

AB - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

KW - Microarray

KW - NF-κB

KW - ROS

KW - TNF

KW - TRAF

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Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Abstract

Keywords

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