TY - JOUR
T1 - Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS
AU - Sasazuki, Tomonari
AU - Okazaki, Tatsuma
AU - Tada, Kurisu
AU - Sakon-Komazawa, Sachiko
AU - Katano, Mitsuo
AU - Tanaka, Masao
AU - Yagita, Hideo
AU - Okumura, Ko
AU - Tominaga, Naoko
AU - Hayashizaki, Yoshihide
AU - Okazaki, Yasushi
AU - Nakano, Hiroyasu
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Culture, Sports, Science and Technology, Grant-in-Aid for Scientific Research (B) from Japan Society for the Promotion of Science, Japan, and by a Grant from Human Frontier Science Program (HFSP).
PY - 2004/7
Y1 - 2004/7
N2 - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.
AB - We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.
KW - Microarray
KW - NF-κB
KW - ROS
KW - TNF
KW - TRAF
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U2 - 10.1016/j.molimm.2004.03.030
DO - 10.1016/j.molimm.2004.03.030
M3 - Article
C2 - 15183933
AN - SCOPUS:2942593988
VL - 41
SP - 547
EP - 551
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 5
ER -