Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

Tomonari Sasazuki, Tatsuma Okazaki, Kurisu Tada, Sachiko Sakon-Komazawa, Mitsuo Katano, Masao Tanaka, Hideo Yagita, Ko Okumura, Naoko Tominaga, Yoshihide Hayashizaki, Yasushi Okazaki, Hiroyasu Nakano

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.

Original languageEnglish
Pages (from-to)547-551
Number of pages5
JournalMolecular Immunology
Volume41
Issue number5
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • Microarray
  • NF-κB
  • ROS
  • TNF
  • TRAF

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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