Genome organization and mRNA structure of Periplaneta fuliginosa densovirus imply alternative splicing involvement in viral gene expression

J. Yamagishi, Y. Hu, J. Zheng, H. Bando

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    We determined the complete nucleotide sequence of an infectious clone of the cockroach small spherical virus (CSSV) genome. Analysis of the genome organization and the predicted viral protein sequences showed clearly that this virus should be classified as a new member of the subfamily Densovirinae, genus Densovirus, and should be designated as PfDNV. However, our data revealed some differences between the gene expression strategies used by PfDNV and other DNVs. An internal promoter, in addition to the promoter (p3) at the genome terminus, was observed at map unit 18 (p18), implying transcriptional regulation of generation of the nonstructural proteins of PfDNV. Furthermore, the structural analysis of cDNAs complementary to mRNAs from the region coding for structural proteins suggested alternative splicing and polyadenylation as means for generation of the structural proteins of PfDNV.

    Original languageEnglish
    Pages (from-to)2111-2124
    Number of pages14
    JournalArchives of virology
    Volume144
    Issue number11
    DOIs
    Publication statusPublished - 1999 Dec 16

    ASJC Scopus subject areas

    • Virology

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