Genome editing of medaka

Satoshi Ansai, Masato Kinoshita

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages175-188
Number of pages14
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1630
ISSN (Print)1064-3745

Keywords

  • CRISPR/Cas
  • Genome editing
  • Heteroduplex mobility assay
  • Medaka
  • Targeted integration
  • Targeted mutagenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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