Genome editing in Zebrafish and Medaka

Atsuo Kawahara, Taijiro Yabe, Satoshi Ansai, Shinji Takada, Masato Kinoshita

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Two small ornamental fish, zebrafish and medaka, have become popular model vertebrates suitable for genetic analysis. A number of mutant fish defective in organogenesis and biological responses have been isolated from chemical mutagenesis screens and forward genetic analyses of these mutants have identified novel essential regulators involved in morphological and physiological processes. Recently, remarkable innovations in genome editing technologies, such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) as well as the clustered interspaced short palindromic repeats (CRISPR)/CRISPRassociated (Cas) system, have enabled us to introduce double-strand DNA breaks at target genomic loci, subsequently leading to the disruption of targeted genes (knockout) or their replacement with homologous fragments (knockin). Here, we summarize the usefulness and application of targeted genome modifications in zebrafish and medaka and their relevance to the basic and medical sciences.

Original languageEnglish
Title of host publicationTargeted Genome Editing Using Site-Specific Nucleases
Subtitle of host publicationZFNs, TALENs, and the CRISPR/Cas9 System
PublisherSpringer Japan
Pages119-131
Number of pages13
ISBN (Electronic)9784431552277
ISBN (Print)9784431552260
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

Keywords

  • CRISPR/Cas9
  • Genome editing
  • Medaka
  • TALEN
  • Zebrafish

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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