Genipin crosslinking of electrodeposited chitosan/gelatin hydrogels for cell culture

Ayako Tamura, Kaoru Hiramoto, Kosuke Ino, Noriko Taira, Yuji Nashimoto, Hitoshi Shiku

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Herein, we describe the crosslinking of electrodeposited chitosan/gelatin hydrogel with genipin for cell culture. First, patterned chitosan/gelatin hydrogels were electrodeposited at 14.0V using a patterned electrode. Next, the hydrogel was crosslinked using genipin, a favorable crosslinking agent with low cytotoxicity; this allowed the hydrogel construct to be preserved in a culture medium for over five days. Moreover, mammalian cells were successfully cultured inside and on the crosslinked hydrogels. The hydrogels will be used for the fabrication of three-dimensional tissue organs.

Original languageEnglish
Pages (from-to)1178-1180
Number of pages3
JournalChemistry Letters
Issue number10
Publication statusPublished - 2019


  • Cell culture
  • Electrodeposition
  • Hydrogels

ASJC Scopus subject areas

  • Chemistry(all)


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