Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect

Kanako Usuda, Yasuaki Wada, Yasuhiro Ishimaru, Takanori Kobayashi, Michiko Takahashi, Hiromi Nakanishi, Yasuo Nagato, Satoshi Mori, Naoko K. Nishizawa

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Nicotianamine (NA), a metal chelator ubiquitous in higher plants, serves as an antihypertensive substance in humans. To engineer a novel antihypertensive rice that contains larger amounts of NA, the barley NA synthase gene, HvNAS1, was introduced into rice via Agrobacterium-mediated transformation. The introduced HvNAS1 was driven by pGluB-1, which induces strong gene expression in the endosperm of rice seeds. The NA content in transgenic rice seeds was up to fourfold greater than that in non-transgenic rice seeds. The Cre/loxP DNA excision (CLX) system was used to remove the selectable marker gene for antibiotic resistance. Furthermore, the transgenic rice was crossed with a cleistogamous mutant to prevent gene transfer via pollen dispersal. These two modifications may minimize public concern with regard to the use of this transgenic rice.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalPlant Biotechnology Journal
Volume7
Issue number1
DOIs
Publication statusPublished - 2009 Jan

Keywords

  • Antihypertensive
  • Cleistogamy
  • Cre/loxP DNA excision system
  • Nicotianamine
  • Rice
  • Transgenic

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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