TY - JOUR
T1 - Genetically engineered mesenchymal stem cells stably expressing green fluorescent protein
AU - Halabian, Raheleh
AU - Mohammadi, Mohamad Hosein
AU - Salimi, Mohammad
AU - Amani, Maryam
AU - Roushande, Amaneh Mohammadi
AU - Aghaipoor, Mahnaz
AU - Amirizadeh, Nasser
AU - Ebrahimi, Majid
AU - Najafabadi, Ali Jahanian
AU - Roudkenar, Mehryar Habibi
PY - 2010
Y1 - 2010
N2 - Objective(s): Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this study was establishment of MSCs expressing GFP. Materials and Methods: MSCs were isolated and characterized by Immunophenotyping. The pEGFP-N1 plasmid was extracted from previously transformed Escherichia. coli cells and transfected into MSCs using FuGENE HD transfection reagent. Stable cells were established in the presence of geneticin. Expression of GFP was detected by RT-PCR, western blot analysis and immunoflorecent microscope. Results: MSCs were successfully isolated and characterized. The MSCs transfected with the pEGFP-N1 plasmid expressed GFP both in mRNA and protein levels while cells transfected with empty vector did not. Conclusion: The results suggested that this engineered cell line will be used in the future studies and can easily be traced in vivo.
AB - Objective(s): Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this study was establishment of MSCs expressing GFP. Materials and Methods: MSCs were isolated and characterized by Immunophenotyping. The pEGFP-N1 plasmid was extracted from previously transformed Escherichia. coli cells and transfected into MSCs using FuGENE HD transfection reagent. Stable cells were established in the presence of geneticin. Expression of GFP was detected by RT-PCR, western blot analysis and immunoflorecent microscope. Results: MSCs were successfully isolated and characterized. The MSCs transfected with the pEGFP-N1 plasmid expressed GFP both in mRNA and protein levels while cells transfected with empty vector did not. Conclusion: The results suggested that this engineered cell line will be used in the future studies and can easily be traced in vivo.
KW - Genetic engineering
KW - Green fluorescent protein
KW - Mesenchymal stem cells
KW - Transfection
UR - http://www.scopus.com/inward/record.url?scp=77952749898&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952749898&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:77952749898
VL - 13
SP - 24
EP - 30
JO - Iranian Journal of Basic Medical Sciences
JF - Iranian Journal of Basic Medical Sciences
SN - 2008-3866
IS - 2 SPRING
ER -