Genetically engineered mesenchymal stem cells stably expressing green fluorescent protein

Raheleh Halabian, Mohamad Hosein Mohammadi, Mohammad Salimi, Maryam Amani, Amaneh Mohammadi Roushande, Mahnaz Aghaipoor, Nasser Amirizadeh, Majid Ebrahimi, Ali Jahanian Najafabadi, Mehryar Habibi Roudkenar

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Objective(s): Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this study was establishment of MSCs expressing GFP. Materials and Methods: MSCs were isolated and characterized by Immunophenotyping. The pEGFP-N1 plasmid was extracted from previously transformed Escherichia. coli cells and transfected into MSCs using FuGENE HD transfection reagent. Stable cells were established in the presence of geneticin. Expression of GFP was detected by RT-PCR, western blot analysis and immunoflorecent microscope. Results: MSCs were successfully isolated and characterized. The MSCs transfected with the pEGFP-N1 plasmid expressed GFP both in mRNA and protein levels while cells transfected with empty vector did not. Conclusion: The results suggested that this engineered cell line will be used in the future studies and can easily be traced in vivo.

    Original languageEnglish
    Pages (from-to)24-30
    Number of pages7
    JournalIranian Journal of Basic Medical Sciences
    Volume13
    Issue number2 SPRING
    Publication statusPublished - 2010

    Keywords

    • Genetic engineering
    • Green fluorescent protein
    • Mesenchymal stem cells
    • Transfection

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Immunology and Microbiology(all)
    • Drug Discovery
    • Clinical Biochemistry

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