Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia

Steven Su, Siew Cheng Phua, Robert Derose, Shuhei Chiba, Keishi Narita, Peter N. Kalugin, Toshiaki Katada, Kenji Kontani, Sen Takeda, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

91 Citations (Scopus)

Abstract

Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.

Original languageEnglish
Pages (from-to)1105-1109
Number of pages5
JournalNature Methods
Volume10
Issue number11
DOIs
Publication statusPublished - 2013 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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