Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia

Steven Su, Siew Cheng Phua, Robert Derose, Shuhei Chiba, Keishi Narita, Peter N. Kalugin, Toshiaki Katada, Kenji Kontani, Sen Takeda, Takanari Inoue

    Research output: Contribution to journalArticlepeer-review

    83 Citations (Scopus)

    Abstract

    Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.

    Original languageEnglish
    Pages (from-to)1105-1109
    Number of pages5
    JournalNature Methods
    Volume10
    Issue number11
    DOIs
    Publication statusPublished - 2013 Nov

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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