Genetic Transformation in Helicobacter pylori

Masataka Tsuda, Mikio Karita, Teruo Nakazawa

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells, with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid‐derived region and the corresponding region of the originally residing plasmid in H. pylori.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume37
Issue number1
DOIs
Publication statusPublished - 1993 Jan
Externally publishedYes

Keywords

  • electroporation
  • helicobacter pylori
  • transformation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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